In Vivo Characterization of Ultrasound Contrast Agents: Microbubble Spectroscopy in a Chicken Embryo

Telli Faez (Corresponding Author), Ilya Skachkov, Michel Versluis, Klazina Kooiman, Nico de Jong

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

The dynamics of coated microbubbles was studied in an in vivo model. Biotinylated lipid-coated microbubbles were prepared in-house and were injected into a chick embryo chorioallantoic membrane (CAM) model on the fifth day of incubation. The microbubbles, ranging between 1.0 and 3.5 μm in diameter, were insonified in the frequency range of 4–7 MHz. Two amplitudes of acoustic pressure were applied: 300 kPa and 400 kPa. The fundamental and subharmonic responses were recorded optically with an ultra-fast camera (Brandaris 128) at 20 million frames per second. A subharmonic response was observed for 44% of the studied bubbles. From the data the frequency of the maximum fundamental and subharmonic response was derived for each individual bubble and resulted in the resonance curves of the microbubbles. All the bubbles showed shell (strain) hardening behavior for a higher acoustic pressure. We conclude that the subharmonic oscillations observed in this study belonged to the transmit at resonance (TR) regime
Original languageEnglish
Pages (from-to)1608-1617
JournalUltrasound in medicine and biology
Volume38
Issue number9
DOIs
Publication statusPublished - 2012

Keywords

  • Chicken embryo
  • Ultrasound contrast agent
  • Microbubbles
  • Subharmonic response
  • In vivo

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