Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1608-1617 |
| Journal | Ultrasound in medicine and biology |
| Volume | 38 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- Chicken embryo
- Ultrasound contrast agent
- Microbubbles
- Subharmonic response
- In vivo
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In Vivo Characterization of Ultrasound Contrast Agents: Microbubble Spectroscopy in a Chicken Embryo. / Faez, Telli (Corresponding Author); Skachkov, Ilya ; Versluis, Michel; Kooiman, Klazina; de Jong, Nico.
In: Ultrasound in medicine and biology, Vol. 38, No. 9, 2012, p. 1608-1617.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - In Vivo Characterization of Ultrasound Contrast Agents: Microbubble Spectroscopy in a Chicken Embryo
AU - Faez, Telli
AU - Skachkov, Ilya
AU - Versluis, Michel
AU - Kooiman, Klazina
AU - de Jong, Nico
PY - 2012
Y1 - 2012
N2 - The dynamics of coated microbubbles was studied in an in vivo model. Biotinylated lipid-coated microbubbles were prepared in-house and were injected into a chick embryo chorioallantoic membrane (CAM) model on the fifth day of incubation. The microbubbles, ranging between 1.0 and 3.5 μm in diameter, were insonified in the frequency range of 4–7 MHz. Two amplitudes of acoustic pressure were applied: 300 kPa and 400 kPa. The fundamental and subharmonic responses were recorded optically with an ultra-fast camera (Brandaris 128) at 20 million frames per second. A subharmonic response was observed for 44% of the studied bubbles. From the data the frequency of the maximum fundamental and subharmonic response was derived for each individual bubble and resulted in the resonance curves of the microbubbles. All the bubbles showed shell (strain) hardening behavior for a higher acoustic pressure. We conclude that the subharmonic oscillations observed in this study belonged to the transmit at resonance (TR) regime
AB - The dynamics of coated microbubbles was studied in an in vivo model. Biotinylated lipid-coated microbubbles were prepared in-house and were injected into a chick embryo chorioallantoic membrane (CAM) model on the fifth day of incubation. The microbubbles, ranging between 1.0 and 3.5 μm in diameter, were insonified in the frequency range of 4–7 MHz. Two amplitudes of acoustic pressure were applied: 300 kPa and 400 kPa. The fundamental and subharmonic responses were recorded optically with an ultra-fast camera (Brandaris 128) at 20 million frames per second. A subharmonic response was observed for 44% of the studied bubbles. From the data the frequency of the maximum fundamental and subharmonic response was derived for each individual bubble and resulted in the resonance curves of the microbubbles. All the bubbles showed shell (strain) hardening behavior for a higher acoustic pressure. We conclude that the subharmonic oscillations observed in this study belonged to the transmit at resonance (TR) regime
KW - Chicken embryo
KW - Ultrasound contrast agent
KW - Microbubbles
KW - Subharmonic response
KW - In vivo
U2 - 10.1016/j.ultrasmedbio.2012.05.014
DO - 10.1016/j.ultrasmedbio.2012.05.014
M3 - Article
VL - 38
SP - 1608
EP - 1617
JO - Ultrasound in medicine and biology
JF - Ultrasound in medicine and biology
SN - 0301-5629
IS - 9
ER -