Incorporation of Human-Platelet-Derived Growth Factor-BB Encapsulated Poly(lactic-co-glycolic acid) Microspheres into 3D CORAGRAF Enhances Osteogenic Differentiation of Mesenchymal Stromal Cells

Saktiswaren Mohan, Hanumantharao Balaji Raghavendran*, Puvanan Karunanithi, Malliga Raman Murali, Sangeetha Vasudevaraj Naveen, Sepehr Talebian, Mohammad Mehrali, Mehdi Mehrali, Elango Natarajan, Chee Ken Chan, Tunku Kamarul

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Tissue engineering aims to generate or facilitate regrowth or healing of damaged tissues by applying a combination of biomaterials, cells, and bioactive signaling molecules. In this regard, growth factors clearly play important roles in regulating cellular fate. However, uncontrolled release of growth factors has been demonstrated to produce severe side effects on the surrounding tissues. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres (MS) incorporated three-dimensional (3D) CORAGRAF scaffolds were engineered to achieve controlled release of platelet-derived growth factor-BB (PDGF-BB) for the differentiation of stem cells within the 3D polymer network. Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy, and microtomography were applied to characterize the fabricated scaffolds. In vitro study revealed that the CORAGRAF-PLGA-PDGF-BB scaffold system enhanced the release of PDGF-BB for the regulation of cell behavior. Stromal cell attachment, viability, release of osteogenic differentiation markers such as osteocalcin, and upregulation of osteogenic gene expression exhibited positive response. Overall, the developed scaffold system was noted to support rapid cell expansion and differentiation of stromal cells into osteogenic cells in vitro for bone tissue engineering applications.

Original languageEnglish
Pages (from-to)9291-9303
Number of pages13
JournalACS Applied Materials and Interfaces
Volume9
Issue number11
DOIs
Publication statusPublished - 22 Mar 2017
Externally publishedYes

Keywords

  • CORAGRAF
  • microsphere
  • osteogenic
  • platelet-derived growth factor
  • PLGA
  • stromal cell

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