Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds

Anne Marijke Leferink, W.J. Hendrikson, Jeroen Rouwkema, Hermanus Bernardus Johannes Karperien, Clemens van Blitterswijk, Lorenzo Moroni

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)
18 Downloads (Pure)

Abstract

In regenerative medicine studies, cell seeding efficiency is not only optimized by changing the chemistry of the biomaterials used as cell culture substrates, but also by altering scaffold geometry, culture and seeding conditions. In this study, the importance of seeding parameters, such as initial cell number, seeding volume, seeding concentration and seeding condition is shown. Human mesenchymal stem cells (hMSCs) were seeded into cylindrically shaped 4 × 3 mm polymeric scaffolds, fabricated by fused deposition modelling. The initial cell number ranged from 5 × 104 to 8 × 105 cells, in volumes varying from 50 µl to 400 µl. To study the effect of seeding conditions, a dynamic system, by means of an agitation plate, was compared with static culture for both scaffolds placed in a well plate or in a confined agarose moulded well. Cell seeding efficiency decreased when seeded with high initial cell numbers, whereas 2 × 105 cells seemed to be an optimal initial cell number in the scaffolds used here. The influence of seeding volume was shown to be dependent on the initial cell number used. By optimizing seeding parameters for each specific culture system, a more efficient use of donor cells can be achieved. Copyright © 2013 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)679-689
Number of pages11
JournalJournal of tissue engineering and regenerative medicine
Volume10
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016

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Scaffolds
Cell Count
Biocompatible Materials
Scaffolds (biology)
Stem cells
Cell culture
Biomaterials
Sepharose
Dynamical systems
Regenerative Medicine
Mesenchymal Stromal Cells
Cell Size
Geometry
Substrates
Cell Culture Techniques

Keywords

  • cell seeding efficiency
  • mesenchymal stromal cells
  • rapid prototyping

Cite this

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title = "Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds",
abstract = "In regenerative medicine studies, cell seeding efficiency is not only optimized by changing the chemistry of the biomaterials used as cell culture substrates, but also by altering scaffold geometry, culture and seeding conditions. In this study, the importance of seeding parameters, such as initial cell number, seeding volume, seeding concentration and seeding condition is shown. Human mesenchymal stem cells (hMSCs) were seeded into cylindrically shaped 4 × 3 mm polymeric scaffolds, fabricated by fused deposition modelling. The initial cell number ranged from 5 × 104 to 8 × 105 cells, in volumes varying from 50 µl to 400 µl. To study the effect of seeding conditions, a dynamic system, by means of an agitation plate, was compared with static culture for both scaffolds placed in a well plate or in a confined agarose moulded well. Cell seeding efficiency decreased when seeded with high initial cell numbers, whereas 2 × 105 cells seemed to be an optimal initial cell number in the scaffolds used here. The influence of seeding volume was shown to be dependent on the initial cell number used. By optimizing seeding parameters for each specific culture system, a more efficient use of donor cells can be achieved. Copyright {\circledC} 2013 John Wiley & Sons, Ltd.",
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Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds. / Leferink, Anne Marijke; Hendrikson, W.J.; Rouwkema, Jeroen; Karperien, Hermanus Bernardus Johannes; van Blitterswijk, Clemens; Moroni, Lorenzo.

In: Journal of tissue engineering and regenerative medicine, Vol. 10, No. 8, 01.08.2016, p. 679-689.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Moroni, Lorenzo

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