Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Research output: Contribution to conferencePaper

Abstract

We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

Original languageEnglish
Pages1381-1383
Number of pages3
Publication statusPublished - 1 Jan 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006 - Tokyo, Japan
Duration: 5 Nov 20069 Nov 2006
Conference number: 10

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006
Abbreviated titleMicroTAS
CountryJapan
CityTokyo
Period5/11/069/11/06

Keywords

  • Apoptosis
  • Cellular interactions
  • PDMS chips
  • Qdots
  • Time-lapse studies

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  • Cite this

    Le Gac, S., Vermes, I., & Van Den Berg, A. (2006). Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining. 1381-1383. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006, Tokyo, Japan.