Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Séverine Le Gac; Istvan Vermes; Albert Van Den Berg

Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Séverine Le Gac, Istvan Vermes, Albert Van Den Berg

Biomedical and Environmental Sensorsystems

Research output: Contribution to conference › Paper › peer-review

Abstract

We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

Original language	English
Pages	1381-1383
Number of pages	3
Publication status	Published - 1 Jan 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006 Conference number: 10

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006
Abbreviated title	MicroTAS
Country/Territory	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

Apoptosis
Cellular interactions
PDMS chips
Qdots
Time-lapse studies

Cite this

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title = "Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining",

abstract = "We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.",

keywords = "Apoptosis, Cellular interactions, PDMS chips, Qdots, Time-lapse studies",

author = "{Le Gac}, S{\'e}verine and Istvan Vermes and {Van Den Berg}, Albert",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "1381--1383",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006, MicroTAS ; Conference date: 05-11-2006 Through 09-11-2006",

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Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining. / Le Gac, Séverine; Vermes, Istvan; Van Den Berg, Albert.
2006. 1381-1383 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

AU - Le Gac, Séverine

AU - Vermes, Istvan

AU - Van Den Berg, Albert

N1 - Conference code: 10

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

AB - We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

KW - Apoptosis

KW - Cellular interactions

KW - PDMS chips

KW - Qdots

KW - Time-lapse studies

UR - http://www.scopus.com/inward/record.url?scp=84902491423&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902491423

SP - 1381

EP - 1383

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Abstract

Conference

Keywords

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