Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Séverine Le Gac; Istvan Vermes; Albert Van Den Berg

Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Biomedical and Environmental Sensorsystems

Research output: Contribution to conference › Paper › peer-review

Abstract

We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

Original language	English
Pages	1381-1383
Number of pages	3
Publication status	Published - 1 Jan 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006 Conference number: 10

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006
Abbreviated title	MicroTAS
Country/Territory	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

Apoptosis
Cellular interactions
PDMS chips
Qdots
Time-lapse studies

Cite this

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title = "Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining",

abstract = "We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.",

keywords = "Apoptosis, Cellular interactions, PDMS chips, Qdots, Time-lapse studies",

author = "\{Le Gac\}, S{\'e}verine and Istvan Vermes and \{Van Den Berg\}, Albert",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "1381--1383",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006, MicroTAS ; Conference date: 05-11-2006 Through 09-11-2006",

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Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining. / Le Gac, Séverine; Vermes, Istvan; Van Den Berg, Albert.
2006. 1381-1383 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

AU - Le Gac, Séverine

AU - Vermes, Istvan

AU - Van Den Berg, Albert

N1 - Conference code: 10

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

AB - We report here on the study of the interactions between apoptotic cells and macrophages by using time-lapse imaging and PDMS microchips. Cells are stained with fluorescent probes, and introduced in a shallow (<20 μm) and small (i.d. 100 μm) microchamber. Time-lapse imaging enabled us to observe three types of interactions or processes. We also developed novel photostable staining agents specific to apoptotic cells and based on Qdots-probes.

KW - Apoptosis

KW - Cellular interactions

KW - PDMS chips

KW - Qdots

KW - Time-lapse studies

UR - https://www.scopus.com/pages/publications/84902491423

M3 - Paper

AN - SCOPUS:84902491423

SP - 1381

EP - 1383

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Influence of apoptotic cells on macrophages and induction of phagocytosis studied in PDMS microchips using photostable staining

Abstract

Conference

Keywords

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