Influence of fluoride in poly(d,l-lactide)/apatite composites on bone formation

Xiaoman Luo, D. Barbieri, G. Passanisi, Huipin Yuan, Joost Dick de Bruijn

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

The influence of fluoride in poly(d,l-lactide)/apatite composites on ectopic bone formation was evaluated in sheep. Nano-apatite powders with different replacement levels of OH groups by fluoride (F) (0% (F0), 50% (F50), 100% (F100), and excessive (F200)) were co-extruded with poly (d,l-lactide) at a weight ratio of 1:1. Fluoride release from the composites (CF0, CF50, CF100, and CF200) was evaluated in vitro and bone formation was assessed after intramuscular implantation in sheep. After 24 weeks in simulated physiological solution, CF0 and CF50 showed negligible fluoride release, whereas it was considerable from the CF100 and CF200 composites. Histology showed that the incidence of de novo bone formation decreased in implants with increasing fluoride content indicating a negative influence of fluoride on ectopic bone formation. Furthermore, a significant decrease in resorption of the high fluoride-content composites and a reduction in the number of multinucleated giant cells were seen. These results show that instead of promoting, the presence of fluoride in poly(d,l-lactide)/apatite composites seemed to suppresses their resorption and osteoinductive potential in non-osseous sites
Original languageEnglish
Pages (from-to)841-852
JournalJournal of biomedical materials research. Part B: Applied biomaterials
Volume103
Issue number4
DOIs
Publication statusPublished - 18 Aug 2015

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Apatites
Apatite
Fluorides
Bone
Composite materials
Histology
dilactide
Powders

Keywords

  • METIS-310253
  • IR-95504

Cite this

Luo, Xiaoman ; Barbieri, D. ; Passanisi, G. ; Yuan, Huipin ; de Bruijn, Joost Dick. / Influence of fluoride in poly(d,l-lactide)/apatite composites on bone formation. In: Journal of biomedical materials research. Part B: Applied biomaterials. 2015 ; Vol. 103, No. 4. pp. 841-852.
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abstract = "The influence of fluoride in poly(d,l-lactide)/apatite composites on ectopic bone formation was evaluated in sheep. Nano-apatite powders with different replacement levels of OH groups by fluoride (F) (0{\%} (F0), 50{\%} (F50), 100{\%} (F100), and excessive (F200)) were co-extruded with poly (d,l-lactide) at a weight ratio of 1:1. Fluoride release from the composites (CF0, CF50, CF100, and CF200) was evaluated in vitro and bone formation was assessed after intramuscular implantation in sheep. After 24 weeks in simulated physiological solution, CF0 and CF50 showed negligible fluoride release, whereas it was considerable from the CF100 and CF200 composites. Histology showed that the incidence of de novo bone formation decreased in implants with increasing fluoride content indicating a negative influence of fluoride on ectopic bone formation. Furthermore, a significant decrease in resorption of the high fluoride-content composites and a reduction in the number of multinucleated giant cells were seen. These results show that instead of promoting, the presence of fluoride in poly(d,l-lactide)/apatite composites seemed to suppresses their resorption and osteoinductive potential in non-osseous sites",
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Influence of fluoride in poly(d,l-lactide)/apatite composites on bone formation. / Luo, Xiaoman; Barbieri, D.; Passanisi, G.; Yuan, Huipin; de Bruijn, Joost Dick.

In: Journal of biomedical materials research. Part B: Applied biomaterials, Vol. 103, No. 4, 18.08.2015, p. 841-852.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Influence of fluoride in poly(d,l-lactide)/apatite composites on bone formation

AU - Luo, Xiaoman

AU - Barbieri, D.

AU - Passanisi, G.

AU - Yuan, Huipin

AU - de Bruijn, Joost Dick

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N2 - The influence of fluoride in poly(d,l-lactide)/apatite composites on ectopic bone formation was evaluated in sheep. Nano-apatite powders with different replacement levels of OH groups by fluoride (F) (0% (F0), 50% (F50), 100% (F100), and excessive (F200)) were co-extruded with poly (d,l-lactide) at a weight ratio of 1:1. Fluoride release from the composites (CF0, CF50, CF100, and CF200) was evaluated in vitro and bone formation was assessed after intramuscular implantation in sheep. After 24 weeks in simulated physiological solution, CF0 and CF50 showed negligible fluoride release, whereas it was considerable from the CF100 and CF200 composites. Histology showed that the incidence of de novo bone formation decreased in implants with increasing fluoride content indicating a negative influence of fluoride on ectopic bone formation. Furthermore, a significant decrease in resorption of the high fluoride-content composites and a reduction in the number of multinucleated giant cells were seen. These results show that instead of promoting, the presence of fluoride in poly(d,l-lactide)/apatite composites seemed to suppresses their resorption and osteoinductive potential in non-osseous sites

AB - The influence of fluoride in poly(d,l-lactide)/apatite composites on ectopic bone formation was evaluated in sheep. Nano-apatite powders with different replacement levels of OH groups by fluoride (F) (0% (F0), 50% (F50), 100% (F100), and excessive (F200)) were co-extruded with poly (d,l-lactide) at a weight ratio of 1:1. Fluoride release from the composites (CF0, CF50, CF100, and CF200) was evaluated in vitro and bone formation was assessed after intramuscular implantation in sheep. After 24 weeks in simulated physiological solution, CF0 and CF50 showed negligible fluoride release, whereas it was considerable from the CF100 and CF200 composites. Histology showed that the incidence of de novo bone formation decreased in implants with increasing fluoride content indicating a negative influence of fluoride on ectopic bone formation. Furthermore, a significant decrease in resorption of the high fluoride-content composites and a reduction in the number of multinucleated giant cells were seen. These results show that instead of promoting, the presence of fluoride in poly(d,l-lactide)/apatite composites seemed to suppresses their resorption and osteoinductive potential in non-osseous sites

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