Influence of the Dentinal Wall on the pH of Sodium Hypochlorite during Root Canal Irrigation

R.G. Macedo, N.P. Herrero, P.R. Wesselink, Michel Versluis, L.W.M. van der Sluis

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)


Introduction: The purpose of this study was to evaluate the influence of dentin on the pH levels of different concentrations of sodium hypochlorite (NaOCl) solutions over time and to evaluate if preconditioning of dentin with 17% EDTA or agitation of the NaOCl solution influences these pH levels. Methods: A novel clinically representative model that scales with the ratio of the irrigant volume to the dentin surface area of a human root canal was used. Three standardized bovine dentin bars (2 2 10 mm) were placed in a plastic test tube. A total of 150 tubes were distributed in 29 groups. In the first experiment, the pH of various NaOCl solutions, with different concentrations (3%, 6%, and 9%) and starting pH levels (5 and 12), was monitored during exposure to dentin between 10 and 300 seconds. In a second experiment, the effect of agitation (45 Hz) and pretreatment of dentin with 17% EDTA on the pH levels of various NaOCl solutions was studied after 30 seconds of exposure to dentin. The short-term chemical stability of the tested solutions was assessed for both the concentration and the pH. Results: The exposure time (P < .001) and concentration of the NaOCl solution (P < .011) significantly influence the pH level after exposure to dentin. However, the change in pH is too small to induce a change in the irrigant antimicrobial/tissue dissolution capacity. Conclusions: Agitation of the irrigant and preconditioning of the dentin did not alter the pH (P > .05). Both the pH 5 and pH 12 solutions were chemically stable for 1 hour. (J Endod 2014;40:1005–1008)
Original languageEnglish
Pages (from-to)1005-1008
Number of pages4
JournalJournal of endodontics
Publication statusPublished - 2014


  • IR-94894
  • METIS-304680


Dive into the research topics of 'Influence of the Dentinal Wall on the pH of Sodium Hypochlorite during Root Canal Irrigation'. Together they form a unique fingerprint.

Cite this