Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate

Liyakat Hamid Mujawar, Johannes G.M. Kuerten, D.P. Siregar, A. van Amerongen, Willem Norde

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)
    83 Downloads (Pure)


    During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain‿ spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60%), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70%).
    Original languageUndefined
    Pages (from-to)19380-19388
    Number of pages9
    JournalRSC advances
    Publication statusPublished - 29 Apr 2014


    • EWI-25003
    • IR-91798
    • METIS-305999

    Cite this