Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate

Liyakat Hamid Mujawar, Johannes G.M. Kuerten, D.P. Siregar, A. van Amerongen, Willem Norde

    Research output: Contribution to journalArticleAcademicpeer-review

    6 Citations (Scopus)
    37 Downloads (Pure)

    Abstract

    During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain‿ spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60%), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70%).
    Original languageUndefined
    Pages (from-to)19380-19388
    Number of pages9
    JournalRSC advances
    Volume4
    DOIs
    Publication statusPublished - 29 Apr 2014

    Keywords

    • EWI-25003
    • IR-91798
    • METIS-305999

    Cite this

    Mujawar, L. H., Kuerten, J. G. M., Siregar, D. P., van Amerongen, A., & Norde, W. (2014). Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate. RSC advances, 4, 19380-19388. https://doi.org/10.1039/c4ra01327a
    Mujawar, Liyakat Hamid ; Kuerten, Johannes G.M. ; Siregar, D.P. ; van Amerongen, A. ; Norde, Willem. / Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate. In: RSC advances. 2014 ; Vol. 4. pp. 19380-19388.
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    title = "Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate",
    abstract = "During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain‿ spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60{\%}), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70{\%}).",
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    author = "Mujawar, {Liyakat Hamid} and Kuerten, {Johannes G.M.} and D.P. Siregar and {van Amerongen}, A. and Willem Norde",
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    Mujawar, LH, Kuerten, JGM, Siregar, DP, van Amerongen, A & Norde, W 2014, 'Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate', RSC advances, vol. 4, pp. 19380-19388. https://doi.org/10.1039/c4ra01327a

    Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate. / Mujawar, Liyakat Hamid; Kuerten, Johannes G.M.; Siregar, D.P.; van Amerongen, A.; Norde, Willem.

    In: RSC advances, Vol. 4, 29.04.2014, p. 19380-19388.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Mujawar, Liyakat Hamid

    AU - Kuerten, Johannes G.M.

    AU - Siregar, D.P.

    AU - van Amerongen, A.

    AU - Norde, Willem

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    N2 - During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain‿ spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60%), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70%).

    AB - During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain‿ spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60%), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70%).

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