Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression

Iwan B. Anwar; Asep Santoso; Eko Saputra; Rifky Ismail; J. Jamari; Emile van der Heide

doi:10.4103/jpbs.JPBS_70_17

Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression

Iwan B. Anwar (Corresponding Author), Asep Santoso, Eko Saputra, Rifky Ismail, J. Jamari, Emile van der Heide

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation.

Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula.

Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period.

Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.

Original language	English
Pages (from-to)	43-47
Number of pages	5
Journal	Journal of Pharmacy and Bioallied Sciences
Volume	10
Issue number	1
DOIs	https://doi.org/10.4103/jpbs.JPBS_70_17
Publication status	Published - 2018

Keywords

Cell viability
Human stem cells
Interleukin-6
UHMWPE

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Anwar, I. B., Santoso, A., Saputra, E., Ismail, R., Jamari, J., & van der Heide, E. (2018). Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression. Journal of Pharmacy and Bioallied Sciences, 10(1), 43-47. https://doi.org/10.4103/jpbs.JPBS_70_17

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title = "Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression",

abstract = "Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation.Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula.Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period.Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.",

keywords = "Cell viability, Human stem cells, Interleukin-6, UHMWPE",

author = "Anwar, {Iwan B.} and Asep Santoso and Eko Saputra and Rifky Ismail and J. Jamari and {van der Heide}, Emile",

year = "2018",

doi = "10.4103/jpbs.JPBS_70_17",

language = "English",

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Anwar, IB, Santoso, A, Saputra, E, Ismail, R, Jamari, J & van der Heide, E 2018, 'Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression', Journal of Pharmacy and Bioallied Sciences, vol. 10, no. 1, pp. 43-47. https://doi.org/10.4103/jpbs.JPBS_70_17

Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression. / Anwar, Iwan B. (Corresponding Author); Santoso, Asep; Saputra, Eko et al.
In: Journal of Pharmacy and Bioallied Sciences, Vol. 10, No. 1, 2018, p. 43-47.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material

T2 - An In Vitro Study on Cell Viability and Interleukin-6 Expression

AU - Anwar, Iwan B.

AU - Santoso, Asep

AU - Saputra, Eko

AU - Ismail, Rifky

AU - Jamari, J.

AU - van der Heide, Emile

PY - 2018

Y1 - 2018

N2 - Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation.Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula.Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period.Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.

AB - Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation.Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula.Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period.Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.

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KW - Interleukin-6

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M3 - Article

SN - 0975-7406

VL - 10

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EP - 47

JO - Journal of Pharmacy and Bioallied Sciences

JF - Journal of Pharmacy and Bioallied Sciences

IS - 1

ER -

Initial Response of Human Bone Marrow-Derived Stem Cells after Contact with Ultrahigh-Molecular-Weight Polyethylene (UHMWPE) Material: An In Vitro Study on Cell Viability and Interleukin-6 Expression

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Keywords

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