Injectable chitosan-based hydrogels for cartilage tissue engineering

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Abstract

Water-soluble chitosan derivatives, chitosan-graft-glycolic acid (GA) and phloretic acid (PA) (CH-GA/PA), were designed to obtain biodegradable injectable chitosan hydrogels through enzymatic crosslinking with horseradish peroxidase (HRP) and H2O2. CH-GA/PA polymers were synthesized by first conjugating glycolic acid (GA) to native chitosan to render the polymer soluble at pH 7.4, and subsequent modification with phloretic acid (PA). The CH-GA43/PA10 with a degree of substitution (DS, defined as the number of substituted NH2 groups per 100 glucopyranose rings of chitosan) of GA of 43 and DS of PA of 10 showed a good solubility at pH values up to 10. Short gelation times (e.g. 10 s at a polymer concentration of 3 wt%), as recorded by the vial tilting method, were observed for the CH-GA43/PA10 hydrogels using HRP and H2O2. It was shown that these hydrogels can be readily degraded by lysozyme. In vitro culturing of chondrocytes in CH-GA43/PA10 hydrogels revealed that after 2 weeks the cells were viable and retained their round shape. These features indicate that CH-GA/PA hydrogels are promising as an artificial extracellular matrix for cartilage tissue engineering
Original languageUndefined
Pages (from-to)2544-2551
JournalBiomaterials
Volume30
Issue number13
DOIs
Publication statusPublished - 2009

Keywords

  • Chitosan
  • Chondrocytes
  • Enzymatic crosslinking
  • Biodegradable
  • Hydrogel
  • METIS-262493
  • IR-82340

Cite this

@article{dde23417b46649888bce33dcae2a3a7a,
title = "Injectable chitosan-based hydrogels for cartilage tissue engineering",
abstract = "Water-soluble chitosan derivatives, chitosan-graft-glycolic acid (GA) and phloretic acid (PA) (CH-GA/PA), were designed to obtain biodegradable injectable chitosan hydrogels through enzymatic crosslinking with horseradish peroxidase (HRP) and H2O2. CH-GA/PA polymers were synthesized by first conjugating glycolic acid (GA) to native chitosan to render the polymer soluble at pH 7.4, and subsequent modification with phloretic acid (PA). The CH-GA43/PA10 with a degree of substitution (DS, defined as the number of substituted NH2 groups per 100 glucopyranose rings of chitosan) of GA of 43 and DS of PA of 10 showed a good solubility at pH values up to 10. Short gelation times (e.g. 10 s at a polymer concentration of 3 wt{\%}), as recorded by the vial tilting method, were observed for the CH-GA43/PA10 hydrogels using HRP and H2O2. It was shown that these hydrogels can be readily degraded by lysozyme. In vitro culturing of chondrocytes in CH-GA43/PA10 hydrogels revealed that after 2 weeks the cells were viable and retained their round shape. These features indicate that CH-GA/PA hydrogels are promising as an artificial extracellular matrix for cartilage tissue engineering",
keywords = "Chitosan, Chondrocytes, Enzymatic crosslinking, Biodegradable, Hydrogel, METIS-262493, IR-82340",
author = "R. Jin and {Moreira Teixeira}, Liliana and Dijkstra, {Pieter J.} and Karperien, {Hermanus Bernardus Johannes} and {van Blitterswijk}, Clemens and Zhiyuan Zhong and Jan Feijen",
year = "2009",
doi = "10.1016/j.biomaterials.2009.01.020",
language = "Undefined",
volume = "30",
pages = "2544--2551",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",
number = "13",

}

Injectable chitosan-based hydrogels for cartilage tissue engineering. / Jin, R.; Moreira Teixeira, Liliana; Dijkstra, Pieter J.; Karperien, Hermanus Bernardus Johannes; van Blitterswijk, Clemens; Zhong, Zhiyuan; Feijen, Jan.

In: Biomaterials, Vol. 30, No. 13, 2009, p. 2544-2551.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Injectable chitosan-based hydrogels for cartilage tissue engineering

AU - Jin, R.

AU - Moreira Teixeira, Liliana

AU - Dijkstra, Pieter J.

AU - Karperien, Hermanus Bernardus Johannes

AU - van Blitterswijk, Clemens

AU - Zhong, Zhiyuan

AU - Feijen, Jan

PY - 2009

Y1 - 2009

N2 - Water-soluble chitosan derivatives, chitosan-graft-glycolic acid (GA) and phloretic acid (PA) (CH-GA/PA), were designed to obtain biodegradable injectable chitosan hydrogels through enzymatic crosslinking with horseradish peroxidase (HRP) and H2O2. CH-GA/PA polymers were synthesized by first conjugating glycolic acid (GA) to native chitosan to render the polymer soluble at pH 7.4, and subsequent modification with phloretic acid (PA). The CH-GA43/PA10 with a degree of substitution (DS, defined as the number of substituted NH2 groups per 100 glucopyranose rings of chitosan) of GA of 43 and DS of PA of 10 showed a good solubility at pH values up to 10. Short gelation times (e.g. 10 s at a polymer concentration of 3 wt%), as recorded by the vial tilting method, were observed for the CH-GA43/PA10 hydrogels using HRP and H2O2. It was shown that these hydrogels can be readily degraded by lysozyme. In vitro culturing of chondrocytes in CH-GA43/PA10 hydrogels revealed that after 2 weeks the cells were viable and retained their round shape. These features indicate that CH-GA/PA hydrogels are promising as an artificial extracellular matrix for cartilage tissue engineering

AB - Water-soluble chitosan derivatives, chitosan-graft-glycolic acid (GA) and phloretic acid (PA) (CH-GA/PA), were designed to obtain biodegradable injectable chitosan hydrogels through enzymatic crosslinking with horseradish peroxidase (HRP) and H2O2. CH-GA/PA polymers were synthesized by first conjugating glycolic acid (GA) to native chitosan to render the polymer soluble at pH 7.4, and subsequent modification with phloretic acid (PA). The CH-GA43/PA10 with a degree of substitution (DS, defined as the number of substituted NH2 groups per 100 glucopyranose rings of chitosan) of GA of 43 and DS of PA of 10 showed a good solubility at pH values up to 10. Short gelation times (e.g. 10 s at a polymer concentration of 3 wt%), as recorded by the vial tilting method, were observed for the CH-GA43/PA10 hydrogels using HRP and H2O2. It was shown that these hydrogels can be readily degraded by lysozyme. In vitro culturing of chondrocytes in CH-GA43/PA10 hydrogels revealed that after 2 weeks the cells were viable and retained their round shape. These features indicate that CH-GA/PA hydrogels are promising as an artificial extracellular matrix for cartilage tissue engineering

KW - Chitosan

KW - Chondrocytes

KW - Enzymatic crosslinking

KW - Biodegradable

KW - Hydrogel

KW - METIS-262493

KW - IR-82340

U2 - 10.1016/j.biomaterials.2009.01.020

DO - 10.1016/j.biomaterials.2009.01.020

M3 - Article

VL - 30

SP - 2544

EP - 2551

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 13

ER -