Inline calibration of spatial light modulators in nonlinear microscopy

Daniël W.S. Cox; Harish Sasikumar; Ivo M. Vellekoop

doi:10.1364/AO.567871

Inline calibration of spatial light modulators in nonlinear microscopy

Daniël W.S. Cox^*
, Harish Sasikumar
, Ivo M. Vellekoop

^*Corresponding author for this work

Biomedical Photonic Imaging

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

7 Downloads (Pure)

Abstract

We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as a calibration measurement and requires no additional hardware components. It is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes and works well even under low-light conditions and with strong photobleaching.

Original language	English
Pages (from-to)	6991-6996
Number of pages	6
Journal	Applied Optics
Volume	64
Issue number	24
DOIs	https://doi.org/10.1364/AO.567871
Publication status	Published - 13 Aug 2025

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title = "Inline calibration of spatial light modulators in nonlinear microscopy",

abstract = "We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as a calibration measurement and requires no additional hardware components. It is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes and works well even under low-light conditions and with strong photobleaching.",

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AU - Cox, Daniël W.S.

AU - Sasikumar, Harish

AU - Vellekoop, Ivo M.

PY - 2025/8/13

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N2 - We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as a calibration measurement and requires no additional hardware components. It is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes and works well even under low-light conditions and with strong photobleaching.

AB - We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as a calibration measurement and requires no additional hardware components. It is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes and works well even under low-light conditions and with strong photobleaching.

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U2 - 10.1364/AO.567871

DO - 10.1364/AO.567871

M3 - Article

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JO - Applied Optics

JF - Applied Optics

IS - 24

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Inline calibration of spatial light modulators in nonlinear microscopy

Abstract

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