Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex

Anna Petoukhova, Wiendelt Steenbergen, F. Morales, R. Graaff, Ed de Jong, J.M. Elstrodt, F.F.M. de Mul, G. Rakhorst

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

To investigate the feasibility of instrument-independent perfusion units for laser Doppler flowmetry, a comparison was performed of two commercial fiberoptic laser Doppler perfusion monitors measuring the same flux situation for two different types of probes. In vivo measurements were performed on the cortex of pig’s kidney, with an ultrasonic arterial flow meter as reference. The flow was mainly varied by internal arterial constriction using a balloon catheter. For each probe, instruments are compared in terms of the ratio of laser Doppler flux and arterial flow. For a given probe, the flux-to-flow ratios of the two instruments show a linear mutual relationship for a wide variety of arterial flows and laser Doppler fluxes. In vitro measurements were performed on an aqueous suspension of polystyrene microspheres. For the probe with interfiber distance 500 μm the ratio of the in vivo fluxes appears to agree within 16% to the value found in vitro, while for the 250-μm probe a difference of 28% was found. For a wide range of fluxes, the in vivo flux values of one instrument can be translated into flux values for the other instrument, in spite of the instrumental differences. This enables the user to render experimental results independent of the specific instrument, thus facilitating multi-center studies.
Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalMicrovascular research
Volume66
Issue number2
DOIs
Publication statusPublished - 2003

Fingerprint

Lasers
Perfusion
Fluxes
Kidney
Equipment and Supplies
Monitoring
Kidney Cortex
Laser-Doppler Flowmetry
Polystyrenes
Microspheres
Constriction
Ultrasonics
Suspensions
Swine
Catheters
Balloons
In Vitro Techniques

Keywords

  • Perfusion units
  • Arterial and mild venous constriction
  • Laser Doppler perfusion monitoring
  • Kidney cortex
  • Ultrasonic arterial flow meter
  • Standardization
  • METIS-212500
  • IR-75413
  • Brownian motion

Cite this

Petoukhova, Anna ; Steenbergen, Wiendelt ; Morales, F. ; Graaff, R. ; de Jong, Ed ; Elstrodt, J.M. ; de Mul, F.F.M. ; Rakhorst, G. / Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex. In: Microvascular research. 2003 ; Vol. 66, No. 2. pp. 83-90.
@article{f400ccd578ff43c99981d618558af057,
title = "Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex",
abstract = "To investigate the feasibility of instrument-independent perfusion units for laser Doppler flowmetry, a comparison was performed of two commercial fiberoptic laser Doppler perfusion monitors measuring the same flux situation for two different types of probes. In vivo measurements were performed on the cortex of pig’s kidney, with an ultrasonic arterial flow meter as reference. The flow was mainly varied by internal arterial constriction using a balloon catheter. For each probe, instruments are compared in terms of the ratio of laser Doppler flux and arterial flow. For a given probe, the flux-to-flow ratios of the two instruments show a linear mutual relationship for a wide variety of arterial flows and laser Doppler fluxes. In vitro measurements were performed on an aqueous suspension of polystyrene microspheres. For the probe with interfiber distance 500 μm the ratio of the in vivo fluxes appears to agree within 16{\%} to the value found in vitro, while for the 250-μm probe a difference of 28{\%} was found. For a wide range of fluxes, the in vivo flux values of one instrument can be translated into flux values for the other instrument, in spite of the instrumental differences. This enables the user to render experimental results independent of the specific instrument, thus facilitating multi-center studies.",
keywords = "Perfusion units, Arterial and mild venous constriction, Laser Doppler perfusion monitoring, Kidney cortex, Ultrasonic arterial flow meter, Standardization, METIS-212500, IR-75413, Brownian motion",
author = "Anna Petoukhova and Wiendelt Steenbergen and F. Morales and R. Graaff and {de Jong}, Ed and J.M. Elstrodt and {de Mul}, F.F.M. and G. Rakhorst",
year = "2003",
doi = "10.1016/S0026-2862(03)00036-0",
language = "English",
volume = "66",
pages = "83--90",
journal = "Microvascular research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "2",

}

Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex. / Petoukhova, Anna; Steenbergen, Wiendelt; Morales, F.; Graaff, R.; de Jong, Ed; Elstrodt, J.M.; de Mul, F.F.M.; Rakhorst, G.

In: Microvascular research, Vol. 66, No. 2, 2003, p. 83-90.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex

AU - Petoukhova, Anna

AU - Steenbergen, Wiendelt

AU - Morales, F.

AU - Graaff, R.

AU - de Jong, Ed

AU - Elstrodt, J.M.

AU - de Mul, F.F.M.

AU - Rakhorst, G.

PY - 2003

Y1 - 2003

N2 - To investigate the feasibility of instrument-independent perfusion units for laser Doppler flowmetry, a comparison was performed of two commercial fiberoptic laser Doppler perfusion monitors measuring the same flux situation for two different types of probes. In vivo measurements were performed on the cortex of pig’s kidney, with an ultrasonic arterial flow meter as reference. The flow was mainly varied by internal arterial constriction using a balloon catheter. For each probe, instruments are compared in terms of the ratio of laser Doppler flux and arterial flow. For a given probe, the flux-to-flow ratios of the two instruments show a linear mutual relationship for a wide variety of arterial flows and laser Doppler fluxes. In vitro measurements were performed on an aqueous suspension of polystyrene microspheres. For the probe with interfiber distance 500 μm the ratio of the in vivo fluxes appears to agree within 16% to the value found in vitro, while for the 250-μm probe a difference of 28% was found. For a wide range of fluxes, the in vivo flux values of one instrument can be translated into flux values for the other instrument, in spite of the instrumental differences. This enables the user to render experimental results independent of the specific instrument, thus facilitating multi-center studies.

AB - To investigate the feasibility of instrument-independent perfusion units for laser Doppler flowmetry, a comparison was performed of two commercial fiberoptic laser Doppler perfusion monitors measuring the same flux situation for two different types of probes. In vivo measurements were performed on the cortex of pig’s kidney, with an ultrasonic arterial flow meter as reference. The flow was mainly varied by internal arterial constriction using a balloon catheter. For each probe, instruments are compared in terms of the ratio of laser Doppler flux and arterial flow. For a given probe, the flux-to-flow ratios of the two instruments show a linear mutual relationship for a wide variety of arterial flows and laser Doppler fluxes. In vitro measurements were performed on an aqueous suspension of polystyrene microspheres. For the probe with interfiber distance 500 μm the ratio of the in vivo fluxes appears to agree within 16% to the value found in vitro, while for the 250-μm probe a difference of 28% was found. For a wide range of fluxes, the in vivo flux values of one instrument can be translated into flux values for the other instrument, in spite of the instrumental differences. This enables the user to render experimental results independent of the specific instrument, thus facilitating multi-center studies.

KW - Perfusion units

KW - Arterial and mild venous constriction

KW - Laser Doppler perfusion monitoring

KW - Kidney cortex

KW - Ultrasonic arterial flow meter

KW - Standardization

KW - METIS-212500

KW - IR-75413

KW - Brownian motion

U2 - 10.1016/S0026-2862(03)00036-0

DO - 10.1016/S0026-2862(03)00036-0

M3 - Article

VL - 66

SP - 83

EP - 90

JO - Microvascular research

JF - Microvascular research

SN - 0026-2862

IS - 2

ER -