Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

M. Brivio; R.H. Fokkens; Willem Verboom; David Reinhoudt; Niels Roelof Tas; M.H. Goedbloed; Albert van den Berg

doi:10.1021/ac020185n

Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

M. Brivio, R.H. Fokkens, Willem Verboom, David Reinhoudt, Niels Roelof Tas, M.H. Goedbloed, Albert van den Berg

Research output: Contribution to journal › Article › Academic › peer-review

101 Citations (Scopus)

1 Downloads (Pure)

Abstract

A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.

Original language	English
Pages (from-to)	3972-3976
Number of pages	5
Journal	Analytical chemistry
Volume	74
Issue number	16
DOIs	https://doi.org/10.1021/ac020185n
Publication status	Published - 2002

Keywords

EWI-12748
IR-62307
METIS-208728

Access to Document

10.1021/ac020185n

http://eprints.eemcs.utwente.nl/secure2/12748/01/JAA_74_3972.pdf

Cite this

@article{af660464667b4425b71d4237e8035f2a,

title = "Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry",

abstract = "A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.",

keywords = "EWI-12748, IR-62307, METIS-208728",

author = "M. Brivio and R.H. Fokkens and Willem Verboom and David Reinhoudt and Tas, {Niels Roelof} and M.H. Goedbloed and {van den Berg}, Albert",

note = "696 ",

year = "2002",

doi = "10.1021/ac020185n",

language = "English",

volume = "74",

pages = "3972--3976",

journal = "Analytical chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "16",

}

TY - JOUR

T1 - Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

AU - Brivio, M.

AU - Fokkens, R.H.

AU - Verboom, Willem

AU - Reinhoudt, David

AU - Tas, Niels Roelof

AU - Goedbloed, M.H.

AU - van den Berg, Albert

N1 - 696

PY - 2002

Y1 - 2002

N2 - A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.

AB - A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.

KW - EWI-12748

KW - IR-62307

KW - METIS-208728

U2 - 10.1021/ac020185n

DO - 10.1021/ac020185n

M3 - Article

SN - 0003-2700

VL - 74

SP - 3972

EP - 3976

JO - Analytical chemistry

JF - Analytical chemistry

IS - 16

ER -

Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

Abstract

Keywords

Access to Document

Fingerprint

Cite this