Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

M. Brivio, R.H. Fokkens, Willem Verboom, David Reinhoudt, Niels Roelof Tas, M.H. Goedbloed, Albert van den Berg

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101 Citations (Scopus)


A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.
Original languageEnglish
Pages (from-to)3972-3976
Number of pages5
JournalAnalytical chemistry
Issue number16
Publication statusPublished - 2002


  • EWI-12748
  • IR-62307
  • METIS-208728


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