Integration of an ISFET with a porous microelectrode for application to protein characterization

J. Luo; K. Kaler; W. Olthuis; P. Bergveld

doi:10.1109/CCECE.1996.548047

Integration of an ISFET with a porous microelectrode for application to protein characterization

J. Luo^*, K. Kaler, W. Olthuis, P. Bergveld

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This paper describes a way of dynamic buffer capacity measurement to characterize proteins, using a coulometric sensor-actuator system. The sensor-actuator is made by integrating an ISFET (ion-sensitive field effect transistor) with a porous microelectrode. Lysozyme and ribonuclease were studied. The results show that the buffer capacity of proteins is both a function of pH and their concentrations. The characters of studied proteins can be distinguished each other from measured curves.

Original language	English
Title of host publication	Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering
Subtitle of host publication	May 26-29, 1996, the University of Calgary
Editors	Terrance J. Malkinson
Publisher	IEEE
Pages	96-99
Number of pages	4
Volume	1
ISBN (Print)	0-7803-3143-5
DOIs	https://doi.org/10.1109/CCECE.1996.548047
Publication status	Published - 1996
Externally published	Yes
Event	Canadian Conference on Electrical and Computer Engineering, CCECE 1996: Glimpse into the 21st Century - The University of Calgary, Calgary, Canada Duration: 26 May 1996 → 29 May 1996

Publication series

Name	Proceedings Canadian Conference on Electrical and Computer Engineering
Publisher	IEEE
Volume	1996
ISSN (Print)	0840-7789

Conference

Conference	Canadian Conference on Electrical and Computer Engineering, CCECE 1996
Abbreviated title	CCECE
Country	Canada
City	Calgary
Period	26/05/96 → 29/05/96

Access to Document

10.1109/CCECE.1996.548047

Luo1996integrationFinal published version, 334 KB

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Luo, J., Kaler, K., Olthuis, W., & Bergveld, P. (1996). Integration of an ISFET with a porous microelectrode for application to protein characterization. In T. J. Malkinson (Ed.), Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering: May 26-29, 1996, the University of Calgary (Vol. 1, pp. 96-99). (Proceedings Canadian Conference on Electrical and Computer Engineering; Vol. 1996). IEEE. https://doi.org/10.1109/CCECE.1996.548047

Luo, J. ; Kaler, K. ; Olthuis, W. ; Bergveld, P. / Integration of an ISFET with a porous microelectrode for application to protein characterization. Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering: May 26-29, 1996, the University of Calgary. editor / Terrance J. Malkinson. Vol. 1 IEEE, 1996. pp. 96-99 (Proceedings Canadian Conference on Electrical and Computer Engineering).

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title = "Integration of an ISFET with a porous microelectrode for application to protein characterization",

abstract = "This paper describes a way of dynamic buffer capacity measurement to characterize proteins, using a coulometric sensor-actuator system. The sensor-actuator is made by integrating an ISFET (ion-sensitive field effect transistor) with a porous microelectrode. Lysozyme and ribonuclease were studied. The results show that the buffer capacity of proteins is both a function of pH and their concentrations. The characters of studied proteins can be distinguished each other from measured curves.",

author = "J. Luo and K. Kaler and W. Olthuis and P. Bergveld",

year = "1996",

doi = "10.1109/CCECE.1996.548047",

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Luo, J, Kaler, K, Olthuis, W & Bergveld, P 1996, Integration of an ISFET with a porous microelectrode for application to protein characterization. in TJ Malkinson (ed.), Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering: May 26-29, 1996, the University of Calgary. vol. 1, Proceedings Canadian Conference on Electrical and Computer Engineering, vol. 1996, IEEE, pp. 96-99, Canadian Conference on Electrical and Computer Engineering, CCECE 1996, Calgary, Canada, 26/05/96. https://doi.org/10.1109/CCECE.1996.548047

Integration of an ISFET with a porous microelectrode for application to protein characterization. / Luo, J.; Kaler, K.; Olthuis, W.; Bergveld, P.

Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering: May 26-29, 1996, the University of Calgary. ed. / Terrance J. Malkinson. Vol. 1 IEEE, 1996. p. 96-99 (Proceedings Canadian Conference on Electrical and Computer Engineering; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AB - This paper describes a way of dynamic buffer capacity measurement to characterize proteins, using a coulometric sensor-actuator system. The sensor-actuator is made by integrating an ISFET (ion-sensitive field effect transistor) with a porous microelectrode. Lysozyme and ribonuclease were studied. The results show that the buffer capacity of proteins is both a function of pH and their concentrations. The characters of studied proteins can be distinguished each other from measured curves.

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Luo J, Kaler K, Olthuis W, Bergveld P. Integration of an ISFET with a porous microelectrode for application to protein characterization. In Malkinson TJ, editor, Conference proceedings 1996 Canadian Conference on Electrical and Computer Engineering: May 26-29, 1996, the University of Calgary. Vol. 1. IEEE. 1996. p. 96-99. (Proceedings Canadian Conference on Electrical and Computer Engineering). https://doi.org/10.1109/CCECE.1996.548047