An integrated system for biohydrogen production and separation was designed, constructed and operated where biohydrogen was fermented by Thermococcus litoralis, a heterotrophic archaebacterium, and a two-step gas separation process was coupled to recover and concentrate hydrogen. A special liquid seal system was built to deliver, compress and collect the laboratory scale, low volume gas mixtures consisting of hydrogen, nitrogen and carbon dioxide. As a result, gas mixture with 73% high hydrogen content was produced by a combination of a porous and a non-porous gas separation membrane.
Bélafi-Bakó, K., Búcsú, D., Pientka, Z., Bálint, B., Herbel, Z., Kovács, K. I., & Wessling, M. (2006). Integration of biohydrogen fermentation and gas separation processes to recover and enrich hydrogen. International journal of hydrogen energy, 31(11), 1490-1495. https://doi.org/10.1016/j.ijhydene.2006.06.022