Interleukin-2 Functionalized Nanocapsules for T Cell-Based Immunotherapy

Stefanie U. Frick, Matthias P. Domogalla, Grit Baier, Frederik R. Wurm, Volker Mailänder, Katharina Landfester*, Kerstin Steinbrink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

A major demand on immunotherapy is the direct interference with specific immune cells in vivo. In contrast to antibody-engineered nanoparticles to control dendritic cells function, targeting of T cells for biomedical applications still remains an obstacle as they disclose reduced endocytic activities. Here, by coupling the cytokine interleukin-2 (IL-2) to the surface of hydroxyethyl starch nanocapsules, we demonstrated a direct and specifc T cell targeting in vitro and in vivo by IL-2 receptor-mediated internalization. For this purpose, defined amounts of azide-functionalized IL-2 were linked to alkyne-functionalized hydroxyethyl starch nanocapsules via copper-free click reactions. In combination with validated quantification of the surface-linked IL-2 with anthracen azide, this method allowed for engineering IL-2-functionalized nanocapsules for an efficient targeting of human and murine T cell populations with various IL-2 receptor affinities. This nanocapsule-mediated technique is a promising strategy for T cell-based immunotherapies and may be translated to other cytokine-related targeting systems.

Original languageEnglish
Pages (from-to)9216-9226
Number of pages11
JournalACS nano
Volume10
Issue number10
DOIs
Publication statusPublished - 25 Oct 2016
Externally publishedYes

Keywords

  • click chemistry
  • human
  • hydroxyethyl starch
  • immunotherapy
  • interleukin-2
  • murine
  • nanocapsules
  • T cells

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