Interpolation method for accurate affinity ranking of arrayed ligand analyte interactions

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

The values of the affinity constants (kd, ka, and KD) that are determined by label-free interaction analysis methods are affected by the ligand density. This article outlines a surface plasmon resonance (SPR) imaging method that yields high-throughput globally fitted affinity ranking values using a 96-plex array. A kinetic titration experiment without a regeneration step has been applied for various coupled antibodies binding to a single antigen. Globally fitted rate (kd and ka) and dissociation equilibrium (KD) constants for various ligand densities and analyte concentrations are exponentially interpolated to the KD at Rmax = 100 RU response level (KDR100).
Original languageEnglish
Pages (from-to)21-23
JournalAnalytical biochemistry
Volume500
DOIs
Publication statusPublished - 13 Feb 2016

Keywords

  • METIS-318827
  • IR-103821

Fingerprint Dive into the research topics of 'Interpolation method for accurate affinity ranking of arrayed ligand analyte interactions'. Together they form a unique fingerprint.

Cite this