Interpolation method for accurate affinity ranking of arrayed ligand analyte interactions

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13 Citations (Scopus)


The values of the affinity constants (kd, ka, and KD) that are determined by label-free interaction analysis methods are affected by the ligand density. This article outlines a surface plasmon resonance (SPR) imaging method that yields high-throughput globally fitted affinity ranking values using a 96-plex array. A kinetic titration experiment without a regeneration step has been applied for various coupled antibodies binding to a single antigen. Globally fitted rate (kd and ka) and dissociation equilibrium (KD) constants for various ligand densities and analyte concentrations are exponentially interpolated to the KD at Rmax = 100 RU response level (KDR100).
Original languageEnglish
Pages (from-to)21-23
JournalAnalytical biochemistry
Publication statusPublished - 13 Feb 2016


  • METIS-318827
  • IR-103821

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