Intracellular distribution of 57Co-bleomycin

A.W. Konings, J.J. Rasker, G.A. Posthuma

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Since the first promising results of Nouel et al. 1972, additional positive experience has been obtained with 57Co-Bleomycin (57Co-BLM) as a tumour-localizing agent. In this preclinical study, mice with transplanted osteosarcoma and lymphosarcoma were used and rats with transplanted rhabdomyosarcoma. 57CoCl2 served as a control substance. 57Co-BLM had concentrated in the tumours with a factor 2 to 10 as compared to the (normal) liver of the animals. No preferential concentration in the tumours was found when 57CoCl2 was used. The highest specific activity of 57Co-BLM (cpm/mg protein) was found in a fraction containing mitochondria and lysosomes. Evidence for a lysosomal localization of this diagnostic compound was obtained from experiments in which the mitochondrial-lysosomal fraction was treated with hypertonic media of different osmolarities. Conditions could be found in which many lysosomes burst while almost all mitochondrial were intact. From these experiments it appeared that the radioactivity in the particles obtained from animals injected wtih 57Co-BLM was released very rapidly. It is concluded that 57Co-BLM is preferentially localized in the heavy lysosomes sedimenting together with most of the mitochondria of the cell and that these structures are more fragile than the light lysosomes.

Original languageEnglish
Pages (from-to)497-512
Number of pages16
JournalCurrent topics in radiation research quarterly
Volume12
Issue number1-4
Publication statusPublished - 1 Jan 1978
Externally publishedYes

Fingerprint Dive into the research topics of 'Intracellular distribution of 57Co-bleomycin'. Together they form a unique fingerprint.

  • Cite this