Intracellular manipulation of chromatin using magnetic nanoparticles

Johannes S. Kanger; Vinod Subramaniam; Roel van Driel

doi:10.1007/s10577-008-1239-1

Intracellular manipulation of chromatin using magnetic nanoparticles

Johannes S. Kanger, Vinod Subramaniam, Roel van Driel

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.

Original language	Undefined
Pages (from-to)	511-522
Number of pages	12
Journal	Chromosome research
Volume	16
Issue number	3
DOIs	https://doi.org/10.1007/s10577-008-1239-1
Publication status	Published - 2008

Keywords

IR-77902
Cell nucleus - chromatin structure - magnetic tweezers - nanoparticles
METIS-247777

Access to Document

10.1007/s10577-008-1239-1

Cite this

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KW - Cell nucleus - chromatin structure - magnetic tweezers - nanoparticles

KW - METIS-247777

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