Abstract
HPLC is one of the most used analytical technologies in life sciences, employed in for example, environmental monitoring,
food quality control, pharmaceutical research and other fields. Although many improvements have been made in the last century in the field of chromatography, still there is a need for better and faster chromatographic separations. One of the
types of separations which needs further improvement is “one-shot proteomics” in which proteins from a cell lysate are fully separated with a single column. Most efforts in improving chromatographic columns is by using smaller particles. This
resulted in the use of particles as small as 1.7 μm in ultra high pressure HPLC. Columns with smaller particles require higher pressures to operate and columns with even smaller particles are not feasible due to pressure limitations of the materials and available pumps. To understand how HPLC can be further improved we have to take a look at the origin of dispersion. The dispersion in a column can be described by the van Deemter theory which states that the dispersion as characterized by the constant H, is characterized by H=A+B/u+C*u, with u the elution velocity and A,B and C constants. This thesis focusses on the reduction of the C-term by enhancing lateral mass-transport.
food quality control, pharmaceutical research and other fields. Although many improvements have been made in the last century in the field of chromatography, still there is a need for better and faster chromatographic separations. One of the
types of separations which needs further improvement is “one-shot proteomics” in which proteins from a cell lysate are fully separated with a single column. Most efforts in improving chromatographic columns is by using smaller particles. This
resulted in the use of particles as small as 1.7 μm in ultra high pressure HPLC. Columns with smaller particles require higher pressures to operate and columns with even smaller particles are not feasible due to pressure limitations of the materials and available pumps. To understand how HPLC can be further improved we have to take a look at the origin of dispersion. The dispersion in a column can be described by the van Deemter theory which states that the dispersion as characterized by the constant H, is characterized by H=A+B/u+C*u, with u the elution velocity and A,B and C constants. This thesis focusses on the reduction of the C-term by enhancing lateral mass-transport.
| Original language | English |
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| Qualification | Doctor of Philosophy |
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| Supervisors/Advisors |
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| Award date | 4 Nov 2022 |
| Place of Publication | Enschede |
| Publisher | |
| Print ISBNs | 978-90-365-5477-0 |
| DOIs | |
| Publication status | Published - 4 Nov 2022 |