Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields

Tjitske Heida; Wim Rutten; Enrico Marani

Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields

Tjitske Heida, Wim Rutten, Enrico Marani

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Cortical rat neurons were trapped by negative dielectrophoresis (DEP) using a planar quadrupole electrode structure. The non-uniform field created by this structure was calculated using a finite element software package. By representing the neuron by a single-shell model the membrane potential induced by the electric field can be estimated. It was investigated whether the physiological state of the trapped neurons can be predicted using this estimation. Experimentally, the physiological state of cortical cells trapped at different amplitudes and frequencies of the input signal was determined using a staining method. The conclusion is that a rough estimate of the minimum frequency and maximum amplitude can be given in order to predict the status of viability of the cells after being dielectrophoretically trapped.

Original language	Undefined
Title of host publication	Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey
Place of Publication	Piscattaway, USA
Publisher	IEEE
Pages	-
Number of pages	4
ISBN (Print)	0-7803-7213-1
Publication status	Published - 25 Oct 2001
Event	23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001 - Istanbul, Turkey Duration: 25 Oct 2001 → 28 Oct 2001 Conference number: 23

Publication series

Name
Publisher	IEEE
Volume	1

Conference

Conference	23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001
Abbreviated title	EMBC
Country/Territory	Turkey
City	Istanbul
Period	25/10/01 → 28/10/01

Keywords

IR-42180
METIS-200704

Access to Document

Investigation_predictability_-_heida

Cite this

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title = "Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields",

abstract = "Cortical rat neurons were trapped by negative dielectrophoresis (DEP) using a planar quadrupole electrode structure. The non-uniform field created by this structure was calculated using a finite element software package. By representing the neuron by a single-shell model the membrane potential induced by the electric field can be estimated. It was investigated whether the physiological state of the trapped neurons can be predicted using this estimation. Experimentally, the physiological state of cortical cells trapped at different amplitudes and frequencies of the input signal was determined using a staining method. The conclusion is that a rough estimate of the minimum frequency and maximum amplitude can be given in order to predict the status of viability of the cells after being dielectrophoretically trapped.",

keywords = "IR-42180, METIS-200704",

author = "Tjitske Heida and Wim Rutten and Enrico Marani",

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year = "2001",

month = oct,

day = "25",

language = "Undefined",

isbn = "0-7803-7213-1",

publisher = "IEEE",

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booktitle = "Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey",

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Heida, T, Rutten, W & Marani, E 2001, Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields. in Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey. IEEE, Piscattaway, USA, pp. -, 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001, Istanbul, Turkey, 25/10/01.

Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields. / Heida, Tjitske; Rutten, Wim; Marani, Enrico.
Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey. Piscattaway, USA: IEEE, 2001. p. -.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Investigation of the prediction of neural cell survival after exposure to non-uniform electric fields

AU - Heida, Tjitske

AU - Rutten, Wim

AU - Marani, Enrico

N1 - Conference code: 23

PY - 2001/10/25

Y1 - 2001/10/25

N2 - Cortical rat neurons were trapped by negative dielectrophoresis (DEP) using a planar quadrupole electrode structure. The non-uniform field created by this structure was calculated using a finite element software package. By representing the neuron by a single-shell model the membrane potential induced by the electric field can be estimated. It was investigated whether the physiological state of the trapped neurons can be predicted using this estimation. Experimentally, the physiological state of cortical cells trapped at different amplitudes and frequencies of the input signal was determined using a staining method. The conclusion is that a rough estimate of the minimum frequency and maximum amplitude can be given in order to predict the status of viability of the cells after being dielectrophoretically trapped.

AB - Cortical rat neurons were trapped by negative dielectrophoresis (DEP) using a planar quadrupole electrode structure. The non-uniform field created by this structure was calculated using a finite element software package. By representing the neuron by a single-shell model the membrane potential induced by the electric field can be estimated. It was investigated whether the physiological state of the trapped neurons can be predicted using this estimation. Experimentally, the physiological state of cortical cells trapped at different amplitudes and frequencies of the input signal was determined using a staining method. The conclusion is that a rough estimate of the minimum frequency and maximum amplitude can be given in order to predict the status of viability of the cells after being dielectrophoretically trapped.

KW - IR-42180

KW - METIS-200704

M3 - Conference contribution

SN - 0-7803-7213-1

SP - -

BT - Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey

PB - IEEE

CY - Piscattaway, USA

T2 - 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001

Y2 - 25 October 2001 through 28 October 2001

ER -