Investigations on vinylene carbonate. VI: Immobilization of alkaline phosphatase onto poly(vinylene carbonate) - jeffamine hydrogel beads

Guohua Chen, Leen van der Does, Adriaan Bantjes

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Hydrogel-like polymers with reactive cyclic carbonate (CCA) groups have been obtained by the cross-linking reaction of poly(vinylene carbonate) (PVCA) with Jeffamines®. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.
Original languageEnglish
Pages (from-to)1189-1198
Number of pages10
JournalJournal of applied polymer science
Volume1993
Issue number48
DOIs
Publication statusPublished - 1993

Keywords

  • METIS-105277
  • IR-71043

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