Ion Concentration Polarization: focusing, separation, extraction and particle porosity differentiation

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

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Abstract

Cardiovascular diseases (CVD) remain the number one cause of death around the world. In contrast to other deadly diseases (e.g. cancer), early diagnosis and treatment of CVD could drastically reduce their mortality rates. Hence a need for a point-of-care device for early diagnosis of CVD exists. The goal of this dissertation is to use ion concentration polarization to focus, separate and extract CVD biomarkers (proteins) out of blood.
Ion concentration polarization is a electrokinetic phenomenon that occurs close to an ion perm-selective material, typically a membrane, when an electric field is applied across it. Due to the flux imbalance between anions and cations through the membrane one side of the membrane becomes depleted of all charged species and the other side becomes enriched, resulting in a concentration polarization across the membrane. The concentration gradient close to the depleted side creates an electric field gradient which is necessary for electrophoretic separation and concentration techniques.
In this thesis we suggest a method for patterning such ion perm-selective membranes using capillary forces and we propose the use of ion concentration polarization phenomenon for the characterization of microparticle porosity. In addition, we demonstrate the selective extraction of concentrated analytes with three different ways: i)Continuous low throughput extraction via pressure application ii) Continuous high throughput extraction via a free flow variant of ion concentration polarization focusing iii)Continuous or on-demand droplet extraction. Finally we suggest that monitoring the electric current in combination with local microchannel width variations can be used as method for automated label free operation of ion concentration polarization focusing.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Eijkel, Jan C.T., Supervisor
  • Segerink, Loes Irene, Co-Supervisor
Award date25 Oct 2019
Print ISBNs978-90-365-4843-4
Electronic ISBNs978-90-365-4843-4
DOIs
Publication statusPublished - 25 Oct 2019

Fingerprint

Porosity
Ions
Polarization
Membranes
Ion selective membranes
Electric fields
Throughput
Electric currents
Biomarkers
Microchannels
Anions
Cations
Labels
Blood
Fluxes
Monitoring
Proteins

Keywords

  • Ion concentration polarization
  • electrokinetic separation
  • Cardiovascular disease

Cite this

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title = "Ion Concentration Polarization: focusing, separation, extraction and particle porosity differentiation",
abstract = "Cardiovascular diseases (CVD) remain the number one cause of death around the world. In contrast to other deadly diseases (e.g. cancer), early diagnosis and treatment of CVD could drastically reduce their mortality rates. Hence a need for a point-of-care device for early diagnosis of CVD exists. The goal of this dissertation is to use ion concentration polarization to focus, separate and extract CVD biomarkers (proteins) out of blood.Ion concentration polarization is a electrokinetic phenomenon that occurs close to an ion perm-selective material, typically a membrane, when an electric field is applied across it. Due to the flux imbalance between anions and cations through the membrane one side of the membrane becomes depleted of all charged species and the other side becomes enriched, resulting in a concentration polarization across the membrane. The concentration gradient close to the depleted side creates an electric field gradient which is necessary for electrophoretic separation and concentration techniques.In this thesis we suggest a method for patterning such ion perm-selective membranes using capillary forces and we propose the use of ion concentration polarization phenomenon for the characterization of microparticle porosity. In addition, we demonstrate the selective extraction of concentrated analytes with three different ways: i)Continuous low throughput extraction via pressure application ii) Continuous high throughput extraction via a free flow variant of ion concentration polarization focusing iii)Continuous or on-demand droplet extraction. Finally we suggest that monitoring the electric current in combination with local microchannel width variations can be used as method for automated label free operation of ion concentration polarization focusing.",
keywords = "Ion concentration polarization, electrokinetic separation, Cardiovascular disease",
author = "Vasileios Papadimitriou",
year = "2019",
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Ion Concentration Polarization : focusing, separation, extraction and particle porosity differentiation. / Papadimitriou, Vasileios .

2019. 198 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

TY - THES

T1 - Ion Concentration Polarization

T2 - focusing, separation, extraction and particle porosity differentiation

AU - Papadimitriou, Vasileios

PY - 2019/10/25

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N2 - Cardiovascular diseases (CVD) remain the number one cause of death around the world. In contrast to other deadly diseases (e.g. cancer), early diagnosis and treatment of CVD could drastically reduce their mortality rates. Hence a need for a point-of-care device for early diagnosis of CVD exists. The goal of this dissertation is to use ion concentration polarization to focus, separate and extract CVD biomarkers (proteins) out of blood.Ion concentration polarization is a electrokinetic phenomenon that occurs close to an ion perm-selective material, typically a membrane, when an electric field is applied across it. Due to the flux imbalance between anions and cations through the membrane one side of the membrane becomes depleted of all charged species and the other side becomes enriched, resulting in a concentration polarization across the membrane. The concentration gradient close to the depleted side creates an electric field gradient which is necessary for electrophoretic separation and concentration techniques.In this thesis we suggest a method for patterning such ion perm-selective membranes using capillary forces and we propose the use of ion concentration polarization phenomenon for the characterization of microparticle porosity. In addition, we demonstrate the selective extraction of concentrated analytes with three different ways: i)Continuous low throughput extraction via pressure application ii) Continuous high throughput extraction via a free flow variant of ion concentration polarization focusing iii)Continuous or on-demand droplet extraction. Finally we suggest that monitoring the electric current in combination with local microchannel width variations can be used as method for automated label free operation of ion concentration polarization focusing.

AB - Cardiovascular diseases (CVD) remain the number one cause of death around the world. In contrast to other deadly diseases (e.g. cancer), early diagnosis and treatment of CVD could drastically reduce their mortality rates. Hence a need for a point-of-care device for early diagnosis of CVD exists. The goal of this dissertation is to use ion concentration polarization to focus, separate and extract CVD biomarkers (proteins) out of blood.Ion concentration polarization is a electrokinetic phenomenon that occurs close to an ion perm-selective material, typically a membrane, when an electric field is applied across it. Due to the flux imbalance between anions and cations through the membrane one side of the membrane becomes depleted of all charged species and the other side becomes enriched, resulting in a concentration polarization across the membrane. The concentration gradient close to the depleted side creates an electric field gradient which is necessary for electrophoretic separation and concentration techniques.In this thesis we suggest a method for patterning such ion perm-selective membranes using capillary forces and we propose the use of ion concentration polarization phenomenon for the characterization of microparticle porosity. In addition, we demonstrate the selective extraction of concentrated analytes with three different ways: i)Continuous low throughput extraction via pressure application ii) Continuous high throughput extraction via a free flow variant of ion concentration polarization focusing iii)Continuous or on-demand droplet extraction. Finally we suggest that monitoring the electric current in combination with local microchannel width variations can be used as method for automated label free operation of ion concentration polarization focusing.

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