Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

F. Abali, M. Stevens, A. G.J. Tibbe, L. W.M.M. Terstappen*, P. N. van der Velde, R. B.M. Schasfoort

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)
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Abstract

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

Original languageEnglish
Pages (from-to)45-47
Number of pages3
JournalAnalytical biochemistry
Volume531
DOIs
Publication statusPublished - 15 Aug 2017

Keywords

  • Antibody/protein excretion
  • EpCAM
  • Hybridoma
  • McSPRInter
  • Microwell
  • Single cells
  • SPR cytometry
  • SPRi

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