Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

F. Abali; M. Stevens; A. G.J. Tibbe; L. W.M.M. Terstappen; P. N. van der Velde; R. B.M. Schasfoort

doi:10.1016/j.ab.2017.05.021

Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

F. Abali, M. Stevens, A. G.J. Tibbe, L. W.M.M. Terstappen^*, P. N. van der Velde, R. B.M. Schasfoort

^*Corresponding author for this work

Medical Cell Biophysics

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

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Abstract

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

Original language	English
Pages (from-to)	45-47
Number of pages	3
Journal	Analytical biochemistry
Volume	531
DOIs	https://doi.org/10.1016/j.ab.2017.05.021
Publication status	Published - 15 Aug 2017

Keywords

Antibody/protein excretion
EpCAM
Hybridoma
McSPRInter
Microwell
Single cells
SPR cytometry
SPRi

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10.1016/j.ab.2017.05.021

1-s2.0-S000326971730218X-mainFinal published version, 936 KBLicence: CC BY

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title = "Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging",

abstract = "Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.",

keywords = "Antibody/protein excretion, EpCAM, Hybridoma, McSPRInter, Microwell, Single cells, SPR cytometry, SPRi",

author = "F. Abali and M. Stevens and Tibbe, {A. G.J.} and Terstappen, {L. W.M.M.} and {van der Velde}, {P. N.} and Schasfoort, {R. B.M.}",

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AU - Abali, F.

AU - Stevens, M.

AU - Tibbe, A. G.J.

AU - Terstappen, L. W.M.M.

AU - van der Velde, P. N.

AU - Schasfoort, R. B.M.

PY - 2017/8/15

Y1 - 2017/8/15

N2 - Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

AB - Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

KW - Antibody/protein excretion

KW - EpCAM

KW - Hybridoma

KW - McSPRInter

KW - Microwell

KW - Single cells

KW - SPR cytometry

KW - SPRi

UR - http://www.scopus.com/inward/record.url?scp=85019590976&partnerID=8YFLogxK

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DO - 10.1016/j.ab.2017.05.021

M3 - Article

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AN - SCOPUS:85019590976

SN - 0003-2697

VL - 531

SP - 45

EP - 47

JO - Analytical biochemistry

JF - Analytical biochemistry

ER -

Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Abstract

Keywords

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