Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)
65 Downloads (Pure)

Abstract

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

Original languageEnglish
Pages (from-to)45-47
Number of pages3
JournalAnalytical biochemistry
Volume531
DOIs
Publication statusPublished - 15 Aug 2017

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Imaging techniques
Punching
Sorting
Cell Separation
Therapeutics
Technology
single cell proteins

Keywords

  • Antibody/protein excretion
  • EpCAM
  • Hybridoma
  • McSPRInter
  • Microwell
  • Single cells
  • SPR cytometry
  • SPRi

Cite this

@article{3c9c72ae8b1746569f2d94ab57728043,
title = "Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging",
abstract = "Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.",
keywords = "Antibody/protein excretion, EpCAM, Hybridoma, McSPRInter, Microwell, Single cells, SPR cytometry, SPRi",
author = "F. Abali and M. Stevens and Tibbe, {A. G.J.} and Terstappen, {L. W.M.M.} and {van der Velde}, {P. N.} and Schasfoort, {R. B.M.}",
year = "2017",
month = "8",
day = "15",
doi = "10.1016/j.ab.2017.05.021",
language = "English",
volume = "531",
pages = "45--47",
journal = "Analytical biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

AU - Abali, F.

AU - Stevens, M.

AU - Tibbe, A. G.J.

AU - Terstappen, L. W.M.M.

AU - van der Velde, P. N.

AU - Schasfoort, R. B.M.

PY - 2017/8/15

Y1 - 2017/8/15

N2 - Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

AB - Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

KW - Antibody/protein excretion

KW - EpCAM

KW - Hybridoma

KW - McSPRInter

KW - Microwell

KW - Single cells

KW - SPR cytometry

KW - SPRi

UR - http://www.scopus.com/inward/record.url?scp=85019590976&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2017.05.021

DO - 10.1016/j.ab.2017.05.021

M3 - Article

VL - 531

SP - 45

EP - 47

JO - Analytical biochemistry

JF - Analytical biochemistry

SN - 0003-2697

ER -