Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System

Thais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G.J. Tibbe, Nikolas Stoecklein, Laura Muinelo-Romay, Roberto Piñeiro, Clotilde Costa*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Tumor heterogeneity has a major role in the development of tumor evasion and resistance to treatments. To study and understand the intrinsic heterogeneity of cancer cells, the use of single-cell isolation technology has had a major boost in recent years, gaining ground to bulk analysis in the study of solid tumors. In the liquid biopsy field, the use of technologies for single-cell analysis has represented a major advance in the study of the heterogeneity of circulating tumor cells (CTCs), providing relevant information about therapy-resistant CTCs. However, single-cell analysis of CTCs is still challenging due to the weakness and scarcity of these cells. In this chapter, we describe a protocol for CTCs isolation at a single-cell level using the VyCAP Puncher system.

Original languageEnglish
Title of host publicationSingle Cell Analysis
Subtitle of host publicationMethods and Protocols
EditorsMiodrag Gužvić
PublisherHumana Press
Number of pages6
ISBN (Electronic)978-1-0716-3621-3
ISBN (Print)978-1-0716-3620-6
Publication statusPublished - 2024
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • NLA
  • CTC
  • Liquid biopsy
  • Puncher
  • Sequencing
  • Single cell
  • Tumor heterogeneity
  • VyCAP
  • CellSearch


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