Key features in the optical properties of tissue during and after radiofrequency ablation

Francis Kalloor Joseph*, Pranav Lanka, Hindrik Kruit, Sanathana Konugolu Venkata Sekar, Andrea Farina, Rinaldo Cubeddu, Srirang Manohar, Antonio Pifferi

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

2 Citations (Scopus)
167 Downloads (Pure)


The change in the optical properties of tissue during thermal treatment can be potentially used to monitor procedures like Radiofrequency Ablation (RFA). We present key features in the optical absorption and scattering of tissue during the RFA procedure and during post-ablation cooling down to room temperature. We have used time-resolved diffuse optical spectroscopy for the measurement of the optical properties of tissue for the wavelengths from 650 to 1100 nm. Ex vivo experiments were conducted using a clinical RFA system on bovine liver tissue. Measurements were performed for two temperatures (70°C and 105°C). The following features were observed in the optical properties. First, there was a decrease in optical absorption and an increase in scattering during the treatment. With overtreatment, the absorption increased for initial part of the spectrum (until 910 nm) and scattering decreased in comparison to normal treatment. Secondly, a redshift of the hemoglobin peak and blue shift around water peak was observed in the optical absorption. Finally, a new peak around 840 nm and a valley around 920 nm appeared with heating. When the tissue was allowed to cool down, most of the changes in the absorption around the water peak partially reversed including the blue shift and the valley around 920 nm. Additionally scattering decreased with cooling. Results show key features in the optical properties of tissue during RFA, the effect of overtreatment and post-treatment cooling in ex vivo tissue. Insights from this study will help in advancing optical methods in monitoring thermal treatment.

Original languageEnglish
Title of host publicationMultiscale Imaging and Spectroscopy
EditorsPaul J. Campagnola, Kristen C. Maitland, Darren M. Roblyer
ISBN (Electronic)9781510631953
ISBN (Print)978-1-5106-3195-3
Publication statusPublished - 2 Mar 2020
EventMultiscale Imaging and Spectroscopy 2020 - San Francisco, United States
Duration: 1 Feb 20202 Feb 2020

Publication series

NameProceedings of SPIE


ConferenceMultiscale Imaging and Spectroscopy 2020
Country/TerritoryUnited States
CitySan Francisco


  • Optical properties
  • Radiofrequency ablation
  • Spectroscopy
  • Thermal treatment
  • thermal treatment
  • spectroscopy
  • radiofrequency ablation


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