Lab-in-a-Cell: a microfluidic chip to study a single living cell

F. Wolbers, Ana Valero, Jurjen Emmelkamp, Willem Engel, Helene Andersson, Albert van den Berg, I. Vermes

Research output: Contribution to conferencePoster

Abstract

Introduction: The application of microchip techniques has really entered life science and has started to serve as a driving force for discovery in cell biology, neurobiology, pharmacology and tissue engineering. As the field of cellomics is expected to become a very important one, a chip to perform single cell analysis will be developed. Therefore, the chip has to consist of a sorting unit, sampling unit and a connection to an analysing unit. Methods: The research is divided in autofluorescence (AF) cell detection, electroporation, needle puncture and picosampling. AF cell detection and localization has been performed on a microfluidic glass chip using granulocytes and red blood cells. For electroporation, cells are trapped and electrodes generating a high electrical field create pores in the cell membrane. Further, siliconnitride microneedles (260nm thick) have been made to analyse needle puncture through a cell membrane. Results: With confocal microscopy, it is possible to sort red blood cells from granulocytes on a microfluidic glass chip on the basis of their intrinsic AF. Further, non-viable cells were distinguished from viable cells due to a lower AF intensity. For the sampling unit, an electroporation device and sharp needles have been made to be able to manipulate a cell. Extraction of cell fluid from oocytes has been performed. Conclusion: Our results show the possibility to manipulate and analyse single living cells on chip. All units have to be organised on one fluidic-chip to be able to perform single cell analysis. Precise control of biochemical cellular environment and analysis of the composition of single cells will lead to Lab-in-a-Cell
Original languageEnglish
Pages-
Publication statusPublished - 22 Apr 2004
Event57e Voorjaarscongres NVKC 2004 - Conferentiecentrum De Werelt, Lunteren, Netherlands
Duration: 22 Apr 200423 Apr 2004

Conference

Conference57e Voorjaarscongres NVKC 2004
CountryNetherlands
CityLunteren
Period22/04/0423/04/04

Keywords

  • METIS-310963
  • IR-96475

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