Abstract
In this article, we present a microfluidic device, consisting of a microchannel structure in PDMS bonded to a glass substrate with recessed platinum electrodes. Our device is capable of successive
high-yield single cell encapsulation in droplets, with additional droplet pairing, fusion, shrinkage and electrofusion of two HL60 cells inside a pico liter droplet. Electrofusion yields still remain low (1.2%), obtained results prove droplets are an interesting platform for efficient high-throughput electrofusion (100-1000 cell s-1) of cells in droplets. The application of this platform is not solely cell fusion, but can perform various other cell-based assays, like single cell analysis and differentiation assays.
Original language | Undefined |
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Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
Place of Publication | San Diego |
Publisher | Chemical and Biochemical Society |
Pages | 424-426 |
Number of pages | 3 |
ISBN (Print) | 978-0-9798064-7-6 |
Publication status | Published - 26 Oct 2014 |
Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - Henry B. Gonzalez Convention Center, San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 Conference number: 18 |
Publication series
Name | MicroTAS 2014 |
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Publisher | Chemical and Biochemical Society |
ISSN (Print) | 1556-5904 |
Conference
Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 |
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Abbreviated title | MicroTAS 2014 |
Country/Territory | United States |
City | San Antonio |
Period | 26/10/14 → 30/10/14 |
Keywords
- EWI-25595
- METIS-309827
- IR-93702