Lab-on-Chip platform for high-yield electrofusion in droplets

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    Abstract

    In this article, we present a microfluidic device, consisting of a microchannel structure in PDMS bonded to a glass substrate with recessed platinum electrodes. Our device is capable of successive high-yield single cell encapsulation in droplets, with additional droplet pairing, fusion, shrinkage and electrofusion of two HL60 cells inside a pico liter droplet. Electrofusion yields still remain low (1.2%), obtained results prove droplets are an interesting platform for efficient high-throughput electrofusion (100-1000 cell s-1) of cells in droplets. The application of this platform is not solely cell fusion, but can perform various other cell-based assays, like single cell analysis and differentiation assays.
    Original languageUndefined
    Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    Place of PublicationSan Diego
    PublisherChemical and Biochemical Society
    Pages424-426
    Number of pages3
    ISBN (Print)978-0-9798064-7-6
    Publication statusPublished - 26 Oct 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - San Antonio, United States
    Duration: 26 Oct 201430 Oct 2014
    Conference number: 18

    Publication series

    NameMicroTAS 2014
    PublisherChemical and Biochemical Society
    ISSN (Print)1556-5904

    Conference

    Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySan Antonio
    Period26/10/1430/10/14

    Keywords

    • EWI-25595
    • METIS-309827
    • IR-93702

    Cite this

    Schoeman, R. M., ter Braak, P. M., Bomer, J. G., & van den Berg, A. (2014). Lab-on-Chip platform for high-yield electrofusion in droplets. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 424-426). (MicroTAS 2014). San Diego: Chemical and Biochemical Society.
    Schoeman, R.M. ; ter Braak, Paulus Martinus ; Bomer, Johan G. ; van den Berg, Albert. / Lab-on-Chip platform for high-yield electrofusion in droplets. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego : Chemical and Biochemical Society, 2014. pp. 424-426 (MicroTAS 2014).
    @inproceedings{f3542478ba8e46629c7b65274c949b55,
    title = "Lab-on-Chip platform for high-yield electrofusion in droplets",
    abstract = "In this article, we present a microfluidic device, consisting of a microchannel structure in PDMS bonded to a glass substrate with recessed platinum electrodes. Our device is capable of successive high-yield single cell encapsulation in droplets, with additional droplet pairing, fusion, shrinkage and electrofusion of two HL60 cells inside a pico liter droplet. Electrofusion yields still remain low (1.2{\%}), obtained results prove droplets are an interesting platform for efficient high-throughput electrofusion (100-1000 cell s-1) of cells in droplets. The application of this platform is not solely cell fusion, but can perform various other cell-based assays, like single cell analysis and differentiation assays.",
    keywords = "EWI-25595, METIS-309827, IR-93702",
    author = "R.M. Schoeman and {ter Braak}, {Paulus Martinus} and Bomer, {Johan G.} and {van den Berg}, Albert",
    year = "2014",
    month = "10",
    day = "26",
    language = "Undefined",
    isbn = "978-0-9798064-7-6",
    series = "MicroTAS 2014",
    publisher = "Chemical and Biochemical Society",
    pages = "424--426",
    booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

    }

    Schoeman, RM, ter Braak, PM, Bomer, JG & van den Berg, A 2014, Lab-on-Chip platform for high-yield electrofusion in droplets. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. MicroTAS 2014, Chemical and Biochemical Society, San Diego, pp. 424-426, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014, San Antonio, United States, 26/10/14.

    Lab-on-Chip platform for high-yield electrofusion in droplets. / Schoeman, R.M.; ter Braak, Paulus Martinus; Bomer, Johan G.; van den Berg, Albert.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego : Chemical and Biochemical Society, 2014. p. 424-426 (MicroTAS 2014).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Lab-on-Chip platform for high-yield electrofusion in droplets

    AU - Schoeman, R.M.

    AU - ter Braak, Paulus Martinus

    AU - Bomer, Johan G.

    AU - van den Berg, Albert

    PY - 2014/10/26

    Y1 - 2014/10/26

    N2 - In this article, we present a microfluidic device, consisting of a microchannel structure in PDMS bonded to a glass substrate with recessed platinum electrodes. Our device is capable of successive high-yield single cell encapsulation in droplets, with additional droplet pairing, fusion, shrinkage and electrofusion of two HL60 cells inside a pico liter droplet. Electrofusion yields still remain low (1.2%), obtained results prove droplets are an interesting platform for efficient high-throughput electrofusion (100-1000 cell s-1) of cells in droplets. The application of this platform is not solely cell fusion, but can perform various other cell-based assays, like single cell analysis and differentiation assays.

    AB - In this article, we present a microfluidic device, consisting of a microchannel structure in PDMS bonded to a glass substrate with recessed platinum electrodes. Our device is capable of successive high-yield single cell encapsulation in droplets, with additional droplet pairing, fusion, shrinkage and electrofusion of two HL60 cells inside a pico liter droplet. Electrofusion yields still remain low (1.2%), obtained results prove droplets are an interesting platform for efficient high-throughput electrofusion (100-1000 cell s-1) of cells in droplets. The application of this platform is not solely cell fusion, but can perform various other cell-based assays, like single cell analysis and differentiation assays.

    KW - EWI-25595

    KW - METIS-309827

    KW - IR-93702

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    SN - 978-0-9798064-7-6

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    BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

    PB - Chemical and Biochemical Society

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    ER -

    Schoeman RM, ter Braak PM, Bomer JG, van den Berg A. Lab-on-Chip platform for high-yield electrofusion in droplets. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego: Chemical and Biochemical Society. 2014. p. 424-426. (MicroTAS 2014).