Label-free cell profiling

Richard B.M. Schasfoort; Arthur E.H. Bentlage; Ivan Stojanovic; Alex van der Kooi; Ellen van der Schoot; Leon W.M.M. Terstappen; Gestur Vidarsson

doi:10.1016/j.ab.2013.04.001

Label-free cell profiling

Richard B.M. Schasfoort^*, Arthur E.H. Bentlage, Ivan Stojanovic, Alex van der Kooi, Ellen van der Schoot, Leon W.M.M. Terstappen, Gestur Vidarsson

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.

Original language	English
Pages (from-to)	4-6
Journal	Analytical biochemistry
Volume	439
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2013.04.001
Publication status	Published - 2013

Keywords

Label free
Cell interaction analysis
Red blood cells
SPR imaging
Multiplex fingerprint

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10.1016/j.ab.2013.04.001

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title = "Label-free cell profiling",

abstract = "A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.",

keywords = "Label free, Cell interaction analysis, Red blood cells, SPR imaging, Multiplex fingerprint",

author = "Schasfoort, {Richard B.M.} and Bentlage, {Arthur E.H.} and Ivan Stojanovic and {van der Kooi}, Alex and {van der Schoot}, Ellen and Terstappen, {Leon W.M.M.} and Gestur Vidarsson",

year = "2013",

doi = "10.1016/j.ab.2013.04.001",

language = "English",

volume = "439",

pages = "4--6",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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T1 - Label-free cell profiling

AU - Schasfoort, Richard B.M.

AU - Bentlage, Arthur E.H.

AU - Stojanovic, Ivan

AU - van der Kooi, Alex

AU - van der Schoot, Ellen

AU - Terstappen, Leon W.M.M.

AU - Vidarsson, Gestur

PY - 2013

Y1 - 2013

N2 - A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.

AB - A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.

KW - Label free

KW - Cell interaction analysis

KW - Red blood cells

KW - SPR imaging

KW - Multiplex fingerprint

U2 - 10.1016/j.ab.2013.04.001

DO - 10.1016/j.ab.2013.04.001

M3 - Article

VL - 439

SP - 4

EP - 6

JO - Analytical biochemistry

JF - Analytical biochemistry

SN - 0003-2697

IS - 1

ER -

Label-free cell profiling

Abstract

Keywords

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