Label-free cell profiling

Richard B.M. Schasfoort*, Arthur E.H. Bentlage, Ivan Stojanovic, Alex van der Kooi, Ellen van der Schoot, Leon W.M.M. Terstappen, Gestur Vidarsson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.
Original languageEnglish
Pages (from-to)4-6
JournalAnalytical biochemistry
Volume439
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Label free
  • Cell interaction analysis
  • Red blood cells
  • SPR imaging
  • Multiplex fingerprint

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