Abstract
A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.
Original language | English |
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Pages (from-to) | 4-6 |
Journal | Analytical biochemistry |
Volume | 439 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- Label free
- Cell interaction analysis
- Red blood cells
- SPR imaging
- Multiplex fingerprint