Label-free, high throughput electrical detection of cells in droplets

Evelien Kemna, Loes Irene Segerink, Mathieu Odijk, F. Wolbers, I. Vermes, Albert van den Berg

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.
    Original languageUndefined
    Title of host publicationThe 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012
    EditorsT. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
    PublisherChemical and Biological Micro Systems Society
    Pages13-15
    Number of pages3
    ISBN (Print)1556-5904
    Publication statusPublished - 29 Oct 2012
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan
    Duration: 28 Oct 20121 Nov 2012
    Conference number: 16

    Publication series

    Name
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
    Abbreviated titleMicroTAS
    CountryJapan
    CityOkinawa
    Period28/10/121/11/12

    Keywords

    • EWI-22714
    • IR-83538
    • METIS-293256

    Cite this

    Kemna, E., Segerink, L. I., Odijk, M., Wolbers, F., Vermes, I., & van den Berg, A. (2012). Label-free, high throughput electrical detection of cells in droplets. In T. Fujii, A. Hibara, S. Takeuchi, & T. Fukuba (Eds.), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012 (pp. 13-15). Chemical and Biological Micro Systems Society.
    Kemna, Evelien ; Segerink, Loes Irene ; Odijk, Mathieu ; Wolbers, F. ; Vermes, I. ; van den Berg, Albert. / Label-free, high throughput electrical detection of cells in droplets. The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. editor / T. Fujii ; A. Hibara ; S. Takeuchi ; T. Fukuba. Chemical and Biological Micro Systems Society, 2012. pp. 13-15
    @inproceedings{7376497cb1de438cb88acbdbc89cc97b,
    title = "Label-free, high throughput electrical detection of cells in droplets",
    abstract = "We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.",
    keywords = "EWI-22714, IR-83538, METIS-293256",
    author = "Evelien Kemna and Segerink, {Loes Irene} and Mathieu Odijk and F. Wolbers and I. Vermes and {van den Berg}, Albert",
    year = "2012",
    month = "10",
    day = "29",
    language = "Undefined",
    isbn = "1556-5904",
    publisher = "Chemical and Biological Micro Systems Society",
    pages = "13--15",
    editor = "T. Fujii and A. Hibara and S. Takeuchi and T. Fukuba",
    booktitle = "The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012",

    }

    Kemna, E, Segerink, LI, Odijk, M, Wolbers, F, Vermes, I & van den Berg, A 2012, Label-free, high throughput electrical detection of cells in droplets. in T Fujii, A Hibara, S Takeuchi & T Fukuba (eds), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. Chemical and Biological Micro Systems Society, pp. 13-15, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, Okinawa, Japan, 28/10/12.

    Label-free, high throughput electrical detection of cells in droplets. / Kemna, Evelien; Segerink, Loes Irene; Odijk, Mathieu; Wolbers, F.; Vermes, I.; van den Berg, Albert.

    The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. ed. / T. Fujii; A. Hibara; S. Takeuchi; T. Fukuba. Chemical and Biological Micro Systems Society, 2012. p. 13-15.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Label-free, high throughput electrical detection of cells in droplets

    AU - Kemna, Evelien

    AU - Segerink, Loes Irene

    AU - Odijk, Mathieu

    AU - Wolbers, F.

    AU - Vermes, I.

    AU - van den Berg, Albert

    PY - 2012/10/29

    Y1 - 2012/10/29

    N2 - We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.

    AB - We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.

    KW - EWI-22714

    KW - IR-83538

    KW - METIS-293256

    M3 - Conference contribution

    SN - 1556-5904

    SP - 13

    EP - 15

    BT - The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012

    A2 - Fujii, T.

    A2 - Hibara, A.

    A2 - Takeuchi, S.

    A2 - Fukuba, T.

    PB - Chemical and Biological Micro Systems Society

    ER -

    Kemna E, Segerink LI, Odijk M, Wolbers F, Vermes I, van den Berg A. Label-free, high throughput electrical detection of cells in droplets. In Fujii T, Hibara A, Takeuchi S, Fukuba T, editors, The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. Chemical and Biological Micro Systems Society. 2012. p. 13-15