Label-free, high throughput electrical detection of cells in droplets

Evelien Kemna; Loes Irene Segerink; Mathieu Odijk; F. Wolbers; I. Vermes; Albert van den Berg

Label-free, high throughput electrical detection of cells in droplets

Evelien Kemna, Loes Irene Segerink, Mathieu Odijk, F. Wolbers, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.

Original language	Undefined
Title of host publication	The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012
Editors	T. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
Publisher	The Chemical and Biological Microsystems Society
Pages	13-15
Number of pages	3
ISBN (Print)	1556-5904
Publication status	Published - 29 Oct 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16

Publication series

Name
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
Abbreviated title	MicroTAS
Country/Territory	Japan
City	Okinawa
Period	28/10/12 → 1/11/12

Keywords

EWI-22714
IR-83538
METIS-293256

Access to Document

http://eprints.eemcs.utwente.nl/secure2/22714/01/1.B1-1.pdf

Cite this

Kemna, E., Segerink, L. I., Odijk, M., Wolbers, F., Vermes, I., & van den Berg, A. (2012). Label-free, high throughput electrical detection of cells in droplets. In T. Fujii, A. Hibara, S. Takeuchi, & T. Fukuba (Eds.), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012 (pp. 13-15). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/22714/01/1.B1-1.pdf

@inproceedings{7376497cb1de438cb88acbdbc89cc97b,

title = "Label-free, high throughput electrical detection of cells in droplets",

abstract = "We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.",

keywords = "EWI-22714, IR-83538, METIS-293256",

author = "Evelien Kemna and Segerink, {Loes Irene} and Mathieu Odijk and F. Wolbers and I. Vermes and {van den Berg}, Albert",

year = "2012",

month = oct,

day = "29",

language = "Undefined",

isbn = "1556-5904",

publisher = "The Chemical and Biological Microsystems Society",

pages = "13--15",

editor = "T. Fujii and A. Hibara and S. Takeuchi and T. Fukuba",

booktitle = "The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012",

note = "null ; Conference date: 28-10-2012 Through 01-11-2012",

}

Kemna, E, Segerink, LI , Odijk, M, Wolbers, F, Vermes, I & van den Berg, A 2012, Label-free, high throughput electrical detection of cells in droplets. in T Fujii, A Hibara, S Takeuchi & T Fukuba (eds), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. The Chemical and Biological Microsystems Society, pp. 13-15, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, Okinawa, Japan, 28/10/12. <http://eprints.eemcs.utwente.nl/secure2/22714/01/1.B1-1.pdf>

Label-free, high throughput electrical detection of cells in droplets. / Kemna, Evelien; Segerink, Loes Irene ; Odijk, Mathieu et al.

The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. ed. / T. Fujii; A. Hibara; S. Takeuchi; T. Fukuba. The Chemical and Biological Microsystems Society, 2012. p. 13-15.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Label-free, high throughput electrical detection of cells in droplets

AU - Kemna, Evelien

AU - Segerink, Loes Irene

AU - Odijk, Mathieu

AU - Wolbers, F.

AU - Vermes, I.

AU - van den Berg, Albert

N1 - Conference code: 16

PY - 2012/10/29

Y1 - 2012/10/29

N2 - We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.

AB - We present a label-free, impedance based cell in droplet detection system. Unlike other work [1,2], this system does not involve the use of valves or expensive labor-intensive cell labeling. The presented platform shows for the first time simple, fast and all electrical discrimination between empty and cell containing droplets. This enables the downstream selection of single-cell containing droplets, while the electrical measurement technique allows for easy integration.

KW - EWI-22714

KW - IR-83538

KW - METIS-293256

M3 - Conference contribution

SN - 1556-5904

SP - 13

EP - 15

BT - The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012

A2 - Fujii, T.

A2 - Hibara, A.

A2 - Takeuchi, S.

A2 - Fukuba, T.

PB - The Chemical and Biological Microsystems Society

Y2 - 28 October 2012 through 1 November 2012

ER -