Labeling strategies for bioassays

C.M. Hempen, U. Karst

    Research output: Contribution to journalArticleAcademic

    59 Citations (Scopus)

    Abstract

    Different labeling strategies for enzymatic assays and immunoassays are reviewed. Techniques which make use of direct detection of a label, e.g. radioimmunoassays, are discussed, as are techniques in which the label is associated with inherent signal amplification. Examples of the latter, e.g. enzyme-linked immunosorbent assays or nanoparticle-label based assays, are presented. Coupling of the bioassays to chromatographic separations adds selectivity but renders the assays more difficult to apply. The advantages and drawbacks of the different analytical principles, including future perspectives, are discussed and compared. Selected applications from clinical, pharmaceutical, and environmental analysis are provided as examples.
    Original languageUndefined
    Pages (from-to)572-583
    JournalAnalytical and bioanalytical chemistry
    Volume384
    Issue number3
    DOIs
    Publication statusPublished - 2006

    Keywords

    • IR-74294

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