This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.
|Number of pages||3|
|Publication status||Published - 9 Oct 2005|
|Event||9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States|
Duration: 9 Oct 2005 → 13 Oct 2005
Conference number: 9
|Conference||9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005|
|Period||9/10/05 → 13/10/05|
- Electro-osmotic flow
- Electrokinetic focusing
- Laminar flow
Kohlheyer, D., Unnikrishnan, S., Schlautmann, S., Besselink, G. A. J., Tudos, A. J., & Schasfoort, R. B. M. (2005). Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. 1337-1339. Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States.