Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

D. Kohlheyer, S. Unnikrishnan, Stefan Schlautmann, G.A.J. Besselink, A.J. Tudos, Richardus B.M. Schasfoort

Research output: Contribution to conferencePaperAcademic

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Abstract

This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.
Original languageUndefined
Pages1337-1339
Number of pages3
Publication statusPublished - 9 Oct 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States
Duration: 9 Oct 200513 Oct 2005
Conference number: 9

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005
Abbreviated titleMicroTAS
CountryUnited States
CityBoston
Period9/10/0513/10/05

Keywords

  • Electro-osmotic flow
  • Electrokinetic focusing
  • EWI-19417
  • Micro-array
  • IR-75839
  • Laminar flow

Cite this

Kohlheyer, D., Unnikrishnan, S., Schlautmann, S., Besselink, G. A. J., Tudos, A. J., & Schasfoort, R. B. M. (2005). Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. 1337-1339. Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States.
Kohlheyer, D. ; Unnikrishnan, S. ; Schlautmann, Stefan ; Besselink, G.A.J. ; Tudos, A.J. ; Schasfoort, Richardus B.M. / Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States.3 p.
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title = "Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing",
abstract = "This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.",
keywords = "Electro-osmotic flow, Electrokinetic focusing, EWI-19417, Micro-array, IR-75839, Laminar flow",
author = "D. Kohlheyer and S. Unnikrishnan and Stefan Schlautmann and G.A.J. Besselink and A.J. Tudos and Schasfoort, {Richardus B.M.}",
note = "The authors acknowledge the Technology Foundation (STW) for funding this project.; null ; Conference date: 09-10-2005 Through 13-10-2005",
year = "2005",
month = "10",
day = "9",
language = "Undefined",
pages = "1337--1339",

}

Kohlheyer, D, Unnikrishnan, S, Schlautmann, S, Besselink, GAJ, Tudos, AJ & Schasfoort, RBM 2005, 'Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing' Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States, 9/10/05 - 13/10/05, pp. 1337-1339.

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. / Kohlheyer, D.; Unnikrishnan, S.; Schlautmann, Stefan; Besselink, G.A.J.; Tudos, A.J.; Schasfoort, Richardus B.M.

2005. 1337-1339 Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States.

Research output: Contribution to conferencePaperAcademic

TY - CONF

T1 - Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

AU - Kohlheyer, D.

AU - Unnikrishnan, S.

AU - Schlautmann, Stefan

AU - Besselink, G.A.J.

AU - Tudos, A.J.

AU - Schasfoort, Richardus B.M.

N1 - The authors acknowledge the Technology Foundation (STW) for funding this project.

PY - 2005/10/9

Y1 - 2005/10/9

N2 - This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.

AB - This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.

KW - Electro-osmotic flow

KW - Electrokinetic focusing

KW - EWI-19417

KW - Micro-array

KW - IR-75839

KW - Laminar flow

M3 - Paper

SP - 1337

EP - 1339

ER -

Kohlheyer D, Unnikrishnan S, Schlautmann S, Besselink GAJ, Tudos AJ, Schasfoort RBM. Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. 2005. Paper presented at 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, United States.