Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

D. Kohlheyer; S. Unnikrishnan; S. Schlautmann; G.A.J. Besselink; A.J. Tudos; R.B.M. Schasfoort

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

D. Kohlheyer
, S. Unnikrishnan
, S. Schlautmann
, G.A.J. Besselink
, A.J. Tudos
, R.B.M. Schasfoort

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

57 Downloads (Pure)

Abstract

This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.

Original language	English
Title of host publication	Proceedings of uTAS 2005
Editors	K.F. Jensen, J. Han, D.J. Harrison, J. Voldman
Place of Publication	San Diego
Publisher	Transducer Research Foundation
Pages	1337-1339
Number of pages	3
ISBN (Print)	0-9743611-1-9
Publication status	Published - 9 Oct 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States Duration: 9 Oct 2005 → 13 Oct 2005 Conference number: 9

Conference

Conference	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005
Abbreviated title	MicroTAS
Country/Territory	United States
City	Boston
Period	9/10/05 → 13/10/05

Keywords

Electro-osmotic flow
Electrokinetic focusing
Micro-array
Laminar flow

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MicroTAS 2005 KohlheyerFinal published version, 179 KB

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing
Kohlheyer, D., Unnikrishnan, S., Schlautmann, S., Besselink, G. A. J., Tudos, A. J. & Schasfoort, R. B. M., 9 Oct 2005. 1 p.
Research output: Contribution to conference › Poster › Other research output

Cite this

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title = "Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing",

abstract = "This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.",

keywords = "Electro-osmotic flow, Electrokinetic focusing, Micro-array, Laminar flow",

author = "D. Kohlheyer and S. Unnikrishnan and S. Schlautmann and G.A.J. Besselink and A.J. Tudos and R.B.M. Schasfoort",

note = "The authors acknowledge the Technology Foundation (STW) for funding this project.; 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, MicroTAS ; Conference date: 09-10-2005 Through 13-10-2005",

year = "2005",

month = oct,

day = "9",

language = "English",

isbn = "0-9743611-1-9",

pages = "1337--1339",

editor = "K.F. Jensen and J. Han and D.J. Harrison and J. Voldman",

booktitle = "Proceedings of uTAS 2005",

publisher = "Transducer Research Foundation",

}

Kohlheyer, D, Unnikrishnan, S, Schlautmann, S, Besselink, GAJ, Tudos, AJ & Schasfoort, RBM 2005, Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. in KF Jensen, J Han, DJ Harrison & J Voldman (eds), Proceedings of uTAS 2005. Transducer Research Foundation, San Diego, pp. 1337-1339, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, Massachusetts, United States, 9/10/05.

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing. / Kohlheyer, D.; Unnikrishnan, S.; Schlautmann, S. et al.
Proceedings of uTAS 2005. ed. / K.F. Jensen; J. Han; D.J. Harrison; J. Voldman. San Diego: Transducer Research Foundation, 2005. p. 1337-1339.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

TY - GEN

T1 - Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

AU - Kohlheyer, D.

AU - Unnikrishnan, S.

AU - Schlautmann, S.

AU - Besselink, G.A.J.

AU - Tudos, A.J.

AU - Schasfoort, R.B.M.

N1 - Conference code: 9

PY - 2005/10/9

Y1 - 2005/10/9

N2 - This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.

AB - This paper describes a method to pattern microarrays in a closed microfluidic device. Two perpendicular laminar flow streams can operate in terms to sequentially coat the surface of a flow-chamber with parallel lanes in two directions. Two perpendicular sample streams can be controlled in position and width by applying electrokinetic focusing, for which each of the two streams is sandwiched between two parallel laminar flow streams containing just a buffer solution. Electroosmotic flow allows a simple chip design without any moving parts being involved. With this device configuration it is possible to define an array of up to 169 spots on a surface area of 1 mm2.

KW - Electro-osmotic flow

KW - Electrokinetic focusing

KW - Micro-array

KW - Laminar flow

M3 - Conference contribution

SN - 0-9743611-1-9

SP - 1337

EP - 1339

BT - Proceedings of uTAS 2005

A2 - Jensen, K.F.

A2 - Han, J.

A2 - Harrison, D.J.

A2 - Voldman, J.

PB - Transducer Research Foundation

CY - San Diego

T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005

Y2 - 9 October 2005 through 13 October 2005

ER -

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

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Keywords

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Research output

Laminar Flow Microarray Patterning by Perpendicular Electrokinetic Focusing

Cite this