Laxity characteristics of normal and pathological murine knee joints in vitro

Gerjo J.V.M. van Osch, Leendert Blankevoort, Peter M. van der Kraan, Bernard Janssen, Edsko Hekman, Rik Huiskes, Wim B. van den Berg

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Abstract

The aim of this study was to validate a device developed previously to measure laxity of murine knee joints and to investigate whether experimentally induced pathological conditions result in measurable laxity. The laxity characteristics of normal murine knee joints were derived from measurements of 25 left knees of normal mice. Reporducible, nonlinear s‐shaped load‐displacement curves were determined, and parameters of anterior‐posterior translation, varus‐valgus rotation, and compliance were calculated from the curves. No differences were found between the left and right knee joints of eight mice. The average displacement between 0.8 N of anterior force and 0.8 N of posterior force was 0.47 ± 0.10 mm. The endpoint compliances for anterior and posterior displacements were 0.16 ± 0.03 and 0.16 ± 0.04 mm/N, respectively. The average rotation between a 4 Nmm valgus moment and a 4 Nmm varus moment was 17.4 ± 3.3°. The endpoint compliances for varsus and valgus rotations were 1.1 ± 0.7 and 1.0 ± 0.3°/Nmm, respectively. Storage of the joints at −70°C had no effect on laxity. We also studied the parameters of laxity after pathology of the knee joint was induced. Zymosan‐induced or antigen‐induced arthritis did not increase laxity of the joint. In an osteoarthritis model induced by injection of collagenase, laxity was markedly increased. In conclusion, laxity in the knees of mice can be measured reproducibly, and changes in the characteristics of laxity due to pathological conditions can be quantified.

Original languageEnglish
Pages (from-to)783-791
Number of pages9
JournalJournal of orthopaedic research
Volume13
Issue number5
DOIs
Publication statusPublished - 1 Jan 1995
Externally publishedYes

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Knee Joint
Compliance
Knee
Joint Instability
Collagenases
Osteoarthritis
Arthritis
Joints
Pathology
Equipment and Supplies
Injections
In Vitro Techniques

Cite this

van Osch, G. J. V. M., Blankevoort, L., van der Kraan, P. M., Janssen, B., Hekman, E., Huiskes, R., & van den Berg, W. B. (1995). Laxity characteristics of normal and pathological murine knee joints in vitro. Journal of orthopaedic research, 13(5), 783-791. https://doi.org/10.1002/jor.1100130519
van Osch, Gerjo J.V.M. ; Blankevoort, Leendert ; van der Kraan, Peter M. ; Janssen, Bernard ; Hekman, Edsko ; Huiskes, Rik ; van den Berg, Wim B. / Laxity characteristics of normal and pathological murine knee joints in vitro. In: Journal of orthopaedic research. 1995 ; Vol. 13, No. 5. pp. 783-791.
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abstract = "The aim of this study was to validate a device developed previously to measure laxity of murine knee joints and to investigate whether experimentally induced pathological conditions result in measurable laxity. The laxity characteristics of normal murine knee joints were derived from measurements of 25 left knees of normal mice. Reporducible, nonlinear s‐shaped load‐displacement curves were determined, and parameters of anterior‐posterior translation, varus‐valgus rotation, and compliance were calculated from the curves. No differences were found between the left and right knee joints of eight mice. The average displacement between 0.8 N of anterior force and 0.8 N of posterior force was 0.47 ± 0.10 mm. The endpoint compliances for anterior and posterior displacements were 0.16 ± 0.03 and 0.16 ± 0.04 mm/N, respectively. The average rotation between a 4 Nmm valgus moment and a 4 Nmm varus moment was 17.4 ± 3.3°. The endpoint compliances for varsus and valgus rotations were 1.1 ± 0.7 and 1.0 ± 0.3°/Nmm, respectively. Storage of the joints at −70°C had no effect on laxity. We also studied the parameters of laxity after pathology of the knee joint was induced. Zymosan‐induced or antigen‐induced arthritis did not increase laxity of the joint. In an osteoarthritis model induced by injection of collagenase, laxity was markedly increased. In conclusion, laxity in the knees of mice can be measured reproducibly, and changes in the characteristics of laxity due to pathological conditions can be quantified.",
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van Osch, GJVM, Blankevoort, L, van der Kraan, PM, Janssen, B, Hekman, E, Huiskes, R & van den Berg, WB 1995, 'Laxity characteristics of normal and pathological murine knee joints in vitro' Journal of orthopaedic research, vol. 13, no. 5, pp. 783-791. https://doi.org/10.1002/jor.1100130519

Laxity characteristics of normal and pathological murine knee joints in vitro. / van Osch, Gerjo J.V.M.; Blankevoort, Leendert; van der Kraan, Peter M.; Janssen, Bernard; Hekman, Edsko; Huiskes, Rik; van den Berg, Wim B.

In: Journal of orthopaedic research, Vol. 13, No. 5, 01.01.1995, p. 783-791.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Osch, Gerjo J.V.M.

AU - Blankevoort, Leendert

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AU - Hekman, Edsko

AU - Huiskes, Rik

AU - van den Berg, Wim B.

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van Osch GJVM, Blankevoort L, van der Kraan PM, Janssen B, Hekman E, Huiskes R et al. Laxity characteristics of normal and pathological murine knee joints in vitro. Journal of orthopaedic research. 1995 Jan 1;13(5):783-791. https://doi.org/10.1002/jor.1100130519