LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study

Gerrit Jan Bakker

Research output: ThesisPhD Thesis - Research external, graduation UT

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Abstract

Leukocytes have the ability to rapidly switch their adhesion in responseto the cell’s physiological environment. How these cells modulate their adhesivenessduring the different stages of their life cycle, has been a centralquestion for decades in immunology. Adhesion regulation has its origin atthe molecular scale, where processes are dynamic, heterogenous and tightlyorchestrated. To unravel these unanswered questions it is therefore of utterimportance to probe biological processes in a time-dependent manner ona molecular scale. With the development of Single Particle Tracking andultimately Single Dye Tracing, it is now possible to follow the mobility ofindividual biomolecules on the plasma membrane of living cells. Observationof mobility of individual biomolecules does not only provide informationabout the local micro-environment of the probed biomolecules, but it alsogives complementary insight into the processes at work at the nanolevel.
The aim of this research has been to investigate the lateral mobility of oneof the most important receptors involved in cell adhesion in the immune system:the integrin receptor LFA-1 (lymphocyte function-associated antigen1; αLß2; CD11a/CD18). By using single molecule techniques we have beenable to deepen our understanding of LFA-1 mobility and its consequences forLFA-1 affinity and avidity regulation on THP-1 monocytes and immaturedendritic cells (imDCs).
Original languageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Garcia Parajo, M.F., Supervisor
  • van Hulst, N.F., Supervisor
  • Cambi, A., Co-Supervisor
Award date8 Jul 2011
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-3209-9
DOIs
Publication statusPublished - 8 Jul 2011

Keywords

  • IR-77611
  • METIS-280730

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