LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study

Gerrit Jan Bakker

Research output: ThesisPhD Thesis - Research external, graduation UT

Abstract

Leukocytes have the ability to rapidly switch their adhesion in responseto the cell’s physiological environment. How these cells modulate their adhesivenessduring the different stages of their life cycle, has been a centralquestion for decades in immunology. Adhesion regulation has its origin atthe molecular scale, where processes are dynamic, heterogenous and tightlyorchestrated. To unravel these unanswered questions it is therefore of utterimportance to probe biological processes in a time-dependent manner ona molecular scale. With the development of Single Particle Tracking andultimately Single Dye Tracing, it is now possible to follow the mobility ofindividual biomolecules on the plasma membrane of living cells. Observationof mobility of individual biomolecules does not only provide informationabout the local micro-environment of the probed biomolecules, but it alsogives complementary insight into the processes at work at the nanolevel.
The aim of this research has been to investigate the lateral mobility of oneof the most important receptors involved in cell adhesion in the immune system:the integrin receptor LFA-1 (lymphocyte function-associated antigen1; αLß2; CD11a/CD18). By using single molecule techniques we have beenable to deepen our understanding of LFA-1 mobility and its consequences forLFA-1 affinity and avidity regulation on THP-1 monocytes and immaturedendritic cells (imDCs).
LanguageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Supervisor
  • Supervisor
  • Co-Supervisor
Award date8 Jul 2011
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-3209-9
DOIs
StatePublished - 8 Jul 2011

Fingerprint

Lymphocyte Function-Associated Antigen-1
Leukocytes
Coloring Agents
Membranes
Biological Phenomena
Allergy and Immunology
Life Cycle Stages
Cell Adhesion
Integrins
Monocytes
Immune System
Cell Membrane
Lymphocytes
Research

Keywords

  • IR-77611
  • METIS-280730

Cite this

Bakker, Gerrit Jan. / LFA-1 dynamics on the membrane of leukocytes : a single dye tracing study. Enschede : University of Twente, 2011. 148 p.
@phdthesis{3f05a498e7b143f0a597f330884c2eaf,
title = "LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study",
abstract = "Leukocytes have the ability to rapidly switch their adhesion in responseto the cell’s physiological environment. How these cells modulate their adhesivenessduring the different stages of their life cycle, has been a centralquestion for decades in immunology. Adhesion regulation has its origin atthe molecular scale, where processes are dynamic, heterogenous and tightlyorchestrated. To unravel these unanswered questions it is therefore of utterimportance to probe biological processes in a time-dependent manner ona molecular scale. With the development of Single Particle Tracking andultimately Single Dye Tracing, it is now possible to follow the mobility ofindividual biomolecules on the plasma membrane of living cells. Observationof mobility of individual biomolecules does not only provide informationabout the local micro-environment of the probed biomolecules, but it alsogives complementary insight into the processes at work at the nanolevel.The aim of this research has been to investigate the lateral mobility of oneof the most important receptors involved in cell adhesion in the immune system:the integrin receptor LFA-1 (lymphocyte function-associated antigen1; αL{\ss}2; CD11a/CD18). By using single molecule techniques we have beenable to deepen our understanding of LFA-1 mobility and its consequences forLFA-1 affinity and avidity regulation on THP-1 monocytes and immaturedendritic cells (imDCs).",
keywords = "IR-77611, METIS-280730",
author = "Bakker, {Gerrit Jan}",
year = "2011",
month = "7",
day = "8",
doi = "10.3990/1.9789036532099",
language = "English",
isbn = "978-90-365-3209-9",
publisher = "University of Twente",
address = "Netherlands",
school = "University of Twente",

}

LFA-1 dynamics on the membrane of leukocytes : a single dye tracing study. / Bakker, Gerrit Jan.

Enschede : University of Twente, 2011. 148 p.

Research output: ThesisPhD Thesis - Research external, graduation UT

TY - THES

T1 - LFA-1 dynamics on the membrane of leukocytes

T2 - a single dye tracing study

AU - Bakker,Gerrit Jan

PY - 2011/7/8

Y1 - 2011/7/8

N2 - Leukocytes have the ability to rapidly switch their adhesion in responseto the cell’s physiological environment. How these cells modulate their adhesivenessduring the different stages of their life cycle, has been a centralquestion for decades in immunology. Adhesion regulation has its origin atthe molecular scale, where processes are dynamic, heterogenous and tightlyorchestrated. To unravel these unanswered questions it is therefore of utterimportance to probe biological processes in a time-dependent manner ona molecular scale. With the development of Single Particle Tracking andultimately Single Dye Tracing, it is now possible to follow the mobility ofindividual biomolecules on the plasma membrane of living cells. Observationof mobility of individual biomolecules does not only provide informationabout the local micro-environment of the probed biomolecules, but it alsogives complementary insight into the processes at work at the nanolevel.The aim of this research has been to investigate the lateral mobility of oneof the most important receptors involved in cell adhesion in the immune system:the integrin receptor LFA-1 (lymphocyte function-associated antigen1; αLß2; CD11a/CD18). By using single molecule techniques we have beenable to deepen our understanding of LFA-1 mobility and its consequences forLFA-1 affinity and avidity regulation on THP-1 monocytes and immaturedendritic cells (imDCs).

AB - Leukocytes have the ability to rapidly switch their adhesion in responseto the cell’s physiological environment. How these cells modulate their adhesivenessduring the different stages of their life cycle, has been a centralquestion for decades in immunology. Adhesion regulation has its origin atthe molecular scale, where processes are dynamic, heterogenous and tightlyorchestrated. To unravel these unanswered questions it is therefore of utterimportance to probe biological processes in a time-dependent manner ona molecular scale. With the development of Single Particle Tracking andultimately Single Dye Tracing, it is now possible to follow the mobility ofindividual biomolecules on the plasma membrane of living cells. Observationof mobility of individual biomolecules does not only provide informationabout the local micro-environment of the probed biomolecules, but it alsogives complementary insight into the processes at work at the nanolevel.The aim of this research has been to investigate the lateral mobility of oneof the most important receptors involved in cell adhesion in the immune system:the integrin receptor LFA-1 (lymphocyte function-associated antigen1; αLß2; CD11a/CD18). By using single molecule techniques we have beenable to deepen our understanding of LFA-1 mobility and its consequences forLFA-1 affinity and avidity regulation on THP-1 monocytes and immaturedendritic cells (imDCs).

KW - IR-77611

KW - METIS-280730

U2 - 10.3990/1.9789036532099

DO - 10.3990/1.9789036532099

M3 - PhD Thesis - Research external, graduation UT

SN - 978-90-365-3209-9

PB - University of Twente

CY - Enschede

ER -

Bakker GJ. LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study. Enschede: University of Twente, 2011. 148 p. Available from, DOI: 10.3990/1.9789036532099