LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study

G.J. Bakker, Gerrit Jan Bakker

Research output: ThesisPhD Thesis - Research external, graduation UT

Abstract

Leukocytes have the ability to rapidly switch their adhesion in response to the cell’s physiological environment. How these cells modulate their adhesiveness during the different stages of their life cycle, has been a central question for decades in immunology. Adhesion regulation has its origin at the molecular scale, where processes are dynamic, heterogenous and tightly orchestrated. To unravel these unanswered questions it is therefore of utter importance to probe biological processes in a time-dependent manner on a molecular scale. With the development of Single Particle Tracking and ultimately Single Dye Tracing, it is now possible to follow the mobility of individual biomolecules on the plasma membrane of living cells. Observation of mobility of individual biomolecules does not only provide information about the local micro-environment of the probed biomolecules, but it also gives complementary insight into the processes at work at the nanolevel. The aim of this research has been to investigate the lateral mobility of one of the most important receptors involved in cell adhesion in the immune system: the integrin receptor LFA-1 (lymphocyte function-associated antigen 1; �Lß2; CD11a/CD18). By using single molecule techniques we have been able to deepen our understanding of LFA-1 mobility and its consequences for LFA-1 affinity and avidity regulation on THP-1 monocytes and immature dendritic cells (imDCs).
LanguageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Supervisor
  • Supervisor
  • Member
  • Schuetz, G.J., Member
  • Kolanus, W., Member
  • Herek, Jennifer Lynn, Member
  • Member
Award date8 Jul 2011
Place of PublicationEnschede
Print ISBNs978-90-365-3209-9
DOIs
StatePublished - 8 Jul 2011

Fingerprint

Lymphocyte Function-Associated Antigen-1
Leukocytes
Coloring Agents
Membranes
Biological Phenomena
Allergy and Immunology
Life Cycle Stages
Cell Adhesion
Integrins
Monocytes
Immune System
Cell Membrane
Lymphocytes
Research

Keywords

  • IR-77611
  • METIS-280730

Cite this

Bakker, G.J. ; Bakker, Gerrit Jan. / LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study. Enschede, 2011. 148 p.
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title = "LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study",
abstract = "Leukocytes have the ability to rapidly switch their adhesion in response to the cell’s physiological environment. How these cells modulate their adhesiveness during the different stages of their life cycle, has been a central question for decades in immunology. Adhesion regulation has its origin at the molecular scale, where processes are dynamic, heterogenous and tightly orchestrated. To unravel these unanswered questions it is therefore of utter importance to probe biological processes in a time-dependent manner on a molecular scale. With the development of Single Particle Tracking and ultimately Single Dye Tracing, it is now possible to follow the mobility of individual biomolecules on the plasma membrane of living cells. Observation of mobility of individual biomolecules does not only provide information about the local micro-environment of the probed biomolecules, but it also gives complementary insight into the processes at work at the nanolevel. The aim of this research has been to investigate the lateral mobility of one of the most important receptors involved in cell adhesion in the immune system: the integrin receptor LFA-1 (lymphocyte function-associated antigen 1; �L{\ss}2; CD11a/CD18). By using single molecule techniques we have been able to deepen our understanding of LFA-1 mobility and its consequences for LFA-1 affinity and avidity regulation on THP-1 monocytes and immature dendritic cells (imDCs).",
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Bakker, GJ & Bakker, GJ 2011, 'LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study', University of Twente, Enschede. DOI: 10.3990/1.9789036532099

LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study. / Bakker, G.J.; Bakker, Gerrit Jan.

Enschede, 2011. 148 p.

Research output: ThesisPhD Thesis - Research external, graduation UT

TY - THES

T1 - LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study

AU - Bakker,G.J.

AU - Bakker,Gerrit Jan

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N2 - Leukocytes have the ability to rapidly switch their adhesion in response to the cell’s physiological environment. How these cells modulate their adhesiveness during the different stages of their life cycle, has been a central question for decades in immunology. Adhesion regulation has its origin at the molecular scale, where processes are dynamic, heterogenous and tightly orchestrated. To unravel these unanswered questions it is therefore of utter importance to probe biological processes in a time-dependent manner on a molecular scale. With the development of Single Particle Tracking and ultimately Single Dye Tracing, it is now possible to follow the mobility of individual biomolecules on the plasma membrane of living cells. Observation of mobility of individual biomolecules does not only provide information about the local micro-environment of the probed biomolecules, but it also gives complementary insight into the processes at work at the nanolevel. The aim of this research has been to investigate the lateral mobility of one of the most important receptors involved in cell adhesion in the immune system: the integrin receptor LFA-1 (lymphocyte function-associated antigen 1; �Lß2; CD11a/CD18). By using single molecule techniques we have been able to deepen our understanding of LFA-1 mobility and its consequences for LFA-1 affinity and avidity regulation on THP-1 monocytes and immature dendritic cells (imDCs).

AB - Leukocytes have the ability to rapidly switch their adhesion in response to the cell’s physiological environment. How these cells modulate their adhesiveness during the different stages of their life cycle, has been a central question for decades in immunology. Adhesion regulation has its origin at the molecular scale, where processes are dynamic, heterogenous and tightly orchestrated. To unravel these unanswered questions it is therefore of utter importance to probe biological processes in a time-dependent manner on a molecular scale. With the development of Single Particle Tracking and ultimately Single Dye Tracing, it is now possible to follow the mobility of individual biomolecules on the plasma membrane of living cells. Observation of mobility of individual biomolecules does not only provide information about the local micro-environment of the probed biomolecules, but it also gives complementary insight into the processes at work at the nanolevel. The aim of this research has been to investigate the lateral mobility of one of the most important receptors involved in cell adhesion in the immune system: the integrin receptor LFA-1 (lymphocyte function-associated antigen 1; �Lß2; CD11a/CD18). By using single molecule techniques we have been able to deepen our understanding of LFA-1 mobility and its consequences for LFA-1 affinity and avidity regulation on THP-1 monocytes and immature dendritic cells (imDCs).

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Bakker GJ, Bakker GJ. LFA-1 dynamics on the membrane of leukocytes: a single dye tracing study. Enschede, 2011. 148 p. Available from, DOI: 10.3990/1.9789036532099