Light-Induced Two-Dimensional FT-IR Spectroscopy of BacterioRhodopsin

P.G.H. Kosters, A.H.B. de Vries, R.P.H. Kooyman

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Abstract

Two-dimensional Fourier transform infrared (2D FT-IR) spectroscopy was applied to study the slower states of the membrane protein bacterioRhodopsin (bR) photocycle, with bR adsorbed on a ZnSe attenuated total reflectance (ATR) crystal. The M and the N states of the bR photocycle could be discriminated, and 2D spectra revealed an N state absorption band at 1536 cm−1 that is normally covered by the broader and bigger 1527 cm−1 M state absorption band. 2D FT-IR absorbance changes are presented in a more convenient way for analysis than in traditional 2D FT-IR spectroscopy by using 2D-phase spectra and phase/amplitude calculations.
Original languageUndefined
Pages (from-to)1659-1664
Number of pages6
JournalApplied spectroscopy
Volume54
Issue number11
DOIs
Publication statusPublished - 2000

Keywords

  • IR-98847
  • METIS-129344

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