Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels

Kjeld G.H. Janssen, J. Li, Jiajie Li, H.T.H. Hoang Thi Hanh, Paul Vulto, Paul Vulto, Richard J.B.H.N. van den Berg, Herman S. Overkleeft, Jan C.T. Eijkel, Niels Roelof Tas, H.J. van der Linden, Heiko J. van der Linden, Thomas Hankemeier

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Abstract

The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.
Original languageUndefined
Pages (from-to)2888-2893
Number of pages6
JournalLab on a chip
Volume12
Issue number16
DOIs
Publication statusPublished - 30 Mar 2012

Keywords

  • EWI-22322
  • IR-81937
  • METIS-296100

Cite this

Janssen, K. G. H., Li, J., Li, J., Hoang Thi Hanh, H. T. H., Vulto, P., Vulto, P., ... Hankemeier, T. (2012). Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels. Lab on a chip, 12(16), 2888-2893. https://doi.org/10.1039/C2LC21011H
Janssen, Kjeld G.H. ; Li, J. ; Li, Jiajie ; Hoang Thi Hanh, H.T.H. ; Vulto, Paul ; Vulto, Paul ; van den Berg, Richard J.B.H.N. ; Overkleeft, Herman S. ; Eijkel, Jan C.T. ; Tas, Niels Roelof ; van der Linden, H.J. ; van der Linden, Heiko J. ; Hankemeier, Thomas. / Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels. In: Lab on a chip. 2012 ; Vol. 12, No. 16. pp. 2888-2893.
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abstract = "The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.",
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author = "Janssen, {Kjeld G.H.} and J. Li and Jiajie Li and {Hoang Thi Hanh}, H.T.H. and Paul Vulto and Paul Vulto and {van den Berg}, {Richard J.B.H.N.} and Overkleeft, {Herman S.} and Eijkel, {Jan C.T.} and Tas, {Niels Roelof} and {van der Linden}, H.J. and {van der Linden}, {Heiko J.} and Thomas Hankemeier",
note = "eemcs-eprint-22322",
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Janssen, KGH, Li, J, Li, J, Hoang Thi Hanh, HTH, Vulto, P, Vulto, P, van den Berg, RJBHN, Overkleeft, HS, Eijkel, JCT, Tas, NR, van der Linden, HJ, van der Linden, HJ & Hankemeier, T 2012, 'Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels' Lab on a chip, vol. 12, no. 16, pp. 2888-2893. https://doi.org/10.1039/C2LC21011H

Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels. / Janssen, Kjeld G.H.; Li, J.; Li, Jiajie; Hoang Thi Hanh, H.T.H.; Vulto, Paul; Vulto, Paul; van den Berg, Richard J.B.H.N.; Overkleeft, Herman S.; Eijkel, Jan C.T.; Tas, Niels Roelof; van der Linden, H.J.; van der Linden, Heiko J.; Hankemeier, Thomas.

In: Lab on a chip, Vol. 12, No. 16, 30.03.2012, p. 2888-2893.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels

AU - Janssen, Kjeld G.H.

AU - Li, J.

AU - Li, Jiajie

AU - Hoang Thi Hanh, H.T.H.

AU - Vulto, Paul

AU - Vulto, Paul

AU - van den Berg, Richard J.B.H.N.

AU - Overkleeft, Herman S.

AU - Eijkel, Jan C.T.

AU - Tas, Niels Roelof

AU - van der Linden, H.J.

AU - van der Linden, Heiko J.

AU - Hankemeier, Thomas

N1 - eemcs-eprint-22322

PY - 2012/3/30

Y1 - 2012/3/30

N2 - The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.

AB - The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.

KW - EWI-22322

KW - IR-81937

KW - METIS-296100

U2 - 10.1039/C2LC21011H

DO - 10.1039/C2LC21011H

M3 - Article

VL - 12

SP - 2888

EP - 2893

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 16

ER -

Janssen KGH, Li J, Li J, Hoang Thi Hanh HTH, Vulto P, Vulto P et al. Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels. Lab on a chip. 2012 Mar 30;12(16):2888-2893. https://doi.org/10.1039/C2LC21011H