Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels

Kjeld G.H. Janssen, J. Li, Jiajie Li, H.T.H. Hoang Thi Hanh, Paul Vulto, Paul Vulto, Richard J.B.H.N. van den Berg, Herman S. Overkleeft, Jan C.T. Eijkel, Niels Roelof Tas, H.J. van der Linden, Heiko J. van der Linden, Thomas Hankemeier

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    The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.
    Original languageUndefined
    Pages (from-to)2888-2893
    Number of pages6
    JournalLab on a chip
    Issue number16
    Publication statusPublished - 30 Mar 2012


    • EWI-22322
    • IR-81937
    • METIS-296100

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