Line-scan Raman microspectrometry for biological applications

C.J. de Grauw, C. Otto, J. Greve

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A high-resolution confocal line-scan Raman microscope was developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolution, for an ideal sample, is 0.5 μm in the lateral direction and 3.5 μm in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.
Original languageEnglish
Pages (from-to)1607-1612
Number of pages6
JournalApplied spectroscopy
Issue number11
Publication statusPublished - 1997


  • Raman
  • Microscopy
  • Resolution
  • Line illumination


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