Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging

Michel M. Teussink, Stanley Lambertus, Frits F. De Mul, Malgorzata B. Rozanowska, Carel B. Hoyng, B. Jeroen Klevering, Thomas Theelen

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    Abstract

    Purpose: Current standards and guidelines aimed at preventing retinal phototoxicity during intentional exposures do not specifically evaluate the contribution of endogenous photosensitizers. However, certain retinal diseases are characterized by abnormal accumulations of potential photosensitizers such as lipofuscin bisretinoids in the retinal pigment epithelium (RPE). We sought to determine these contributions by a numerical assessment of in-vivo photo-oxidative stress during irradiation of RPE lipofuscin.

    Methods: Based on the literature, we calculated the retinal exposure levels, optical filtering of incident radiation by the ocular lens, media, photoreceptors, and RPE melanin, light absorption by lipofuscin, and photochemical effects in the RPE in two situations: exposure to short-wavelength (λ = 488 nm) fundus autofluorescence (SW-AF) excitation light and exposure to indirect (diffuse) sunlight. Results In healthy persons at age 20, 40, and 60, respectively, the rate of oxygen photoconsumption by lipofuscin increases by 1.3, 1.7, and 2.4 fold during SW-AF-imaging as compared to diffuse sunlight. In patients with STGD1 below the age of 30, this rate was 3.3-fold higher compared to age-matched controls during either sunlight or SW-AF imaging.

    Conclusions: Our results suggest that the RPE of patients with STGD1 is generally at increased risk of photo-oxidative stress, while exposure during SW-AF-imaging amplifies this risk. These theoretical results have not yet been verified with in-vivo data due to a lack of sufficiently sensitive in-vivo measurement techniques.

    Original languageEnglish
    Article numbere0172635
    JournalPLoS ONE
    Volume12
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2017

    Fingerprint

    Lipofuscin
    Oxidative stress
    Retinal Pigments
    Retinal Pigment Epithelium
    Optical Imaging
    Oxidative Stress
    oxidative stress
    epithelium
    pigments
    image analysis
    Sunlight
    Imaging techniques
    solar radiation
    Photosensitizing Agents
    standards and grades
    phototoxicity
    eye lens
    Phototoxic Dermatitis
    retinal diseases
    Retinal Diseases

    Cite this

    Teussink, M. M., Lambertus, S., De Mul, F. F., Rozanowska, M. B., Hoyng, C. B., Klevering, B. J., & Theelen, T. (2017). Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging. PLoS ONE, 12(2), [e0172635]. https://doi.org/10.1371/journal.pone.0172635
    Teussink, Michel M. ; Lambertus, Stanley ; De Mul, Frits F. ; Rozanowska, Malgorzata B. ; Hoyng, Carel B. ; Klevering, B. Jeroen ; Theelen, Thomas. / Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging. In: PLoS ONE. 2017 ; Vol. 12, No. 2.
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    abstract = "Purpose: Current standards and guidelines aimed at preventing retinal phototoxicity during intentional exposures do not specifically evaluate the contribution of endogenous photosensitizers. However, certain retinal diseases are characterized by abnormal accumulations of potential photosensitizers such as lipofuscin bisretinoids in the retinal pigment epithelium (RPE). We sought to determine these contributions by a numerical assessment of in-vivo photo-oxidative stress during irradiation of RPE lipofuscin.Methods: Based on the literature, we calculated the retinal exposure levels, optical filtering of incident radiation by the ocular lens, media, photoreceptors, and RPE melanin, light absorption by lipofuscin, and photochemical effects in the RPE in two situations: exposure to short-wavelength (λ = 488 nm) fundus autofluorescence (SW-AF) excitation light and exposure to indirect (diffuse) sunlight. Results In healthy persons at age 20, 40, and 60, respectively, the rate of oxygen photoconsumption by lipofuscin increases by 1.3, 1.7, and 2.4 fold during SW-AF-imaging as compared to diffuse sunlight. In patients with STGD1 below the age of 30, this rate was 3.3-fold higher compared to age-matched controls during either sunlight or SW-AF imaging.Conclusions: Our results suggest that the RPE of patients with STGD1 is generally at increased risk of photo-oxidative stress, while exposure during SW-AF-imaging amplifies this risk. These theoretical results have not yet been verified with in-vivo data due to a lack of sufficiently sensitive in-vivo measurement techniques.",
    author = "Teussink, {Michel M.} and Stanley Lambertus and {De Mul}, {Frits F.} and Rozanowska, {Malgorzata B.} and Hoyng, {Carel B.} and Klevering, {B. Jeroen} and Thomas Theelen",
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    Teussink, MM, Lambertus, S, De Mul, FF, Rozanowska, MB, Hoyng, CB, Klevering, BJ & Theelen, T 2017, 'Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging' PLoS ONE, vol. 12, no. 2, e0172635. https://doi.org/10.1371/journal.pone.0172635

    Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging. / Teussink, Michel M.; Lambertus, Stanley; De Mul, Frits F.; Rozanowska, Malgorzata B.; Hoyng, Carel B.; Klevering, B. Jeroen; Theelen, Thomas.

    In: PLoS ONE, Vol. 12, No. 2, e0172635, 01.02.2017.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Teussink, Michel M.

    AU - Lambertus, Stanley

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    AU - Hoyng, Carel B.

    AU - Klevering, B. Jeroen

    AU - Theelen, Thomas

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    N2 - Purpose: Current standards and guidelines aimed at preventing retinal phototoxicity during intentional exposures do not specifically evaluate the contribution of endogenous photosensitizers. However, certain retinal diseases are characterized by abnormal accumulations of potential photosensitizers such as lipofuscin bisretinoids in the retinal pigment epithelium (RPE). We sought to determine these contributions by a numerical assessment of in-vivo photo-oxidative stress during irradiation of RPE lipofuscin.Methods: Based on the literature, we calculated the retinal exposure levels, optical filtering of incident radiation by the ocular lens, media, photoreceptors, and RPE melanin, light absorption by lipofuscin, and photochemical effects in the RPE in two situations: exposure to short-wavelength (λ = 488 nm) fundus autofluorescence (SW-AF) excitation light and exposure to indirect (diffuse) sunlight. Results In healthy persons at age 20, 40, and 60, respectively, the rate of oxygen photoconsumption by lipofuscin increases by 1.3, 1.7, and 2.4 fold during SW-AF-imaging as compared to diffuse sunlight. In patients with STGD1 below the age of 30, this rate was 3.3-fold higher compared to age-matched controls during either sunlight or SW-AF imaging.Conclusions: Our results suggest that the RPE of patients with STGD1 is generally at increased risk of photo-oxidative stress, while exposure during SW-AF-imaging amplifies this risk. These theoretical results have not yet been verified with in-vivo data due to a lack of sufficiently sensitive in-vivo measurement techniques.

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    Teussink MM, Lambertus S, De Mul FF, Rozanowska MB, Hoyng CB, Klevering BJ et al. Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging. PLoS ONE. 2017 Feb 1;12(2). e0172635. https://doi.org/10.1371/journal.pone.0172635