Liquid chromatography with on-line electrochemical derivatization and fluorescence detection for the determination of phenols

Jörg Meyer, A. Liesener, S. Götz, H. Hayen, U. Karst

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    Abstract

    A new methodological approach for the determination of monosubstituted phenols is described. After liquid chromatographic separation of the analytes, an on-line electrochemical derivatization is carried out and the reaction products are detected fluorometrically. Phenols are oxidized in the electrochemical cell to form fluorescent dimers and higher oligomers, which were identified by on-line electrochemistry/mass spectrometry. Major advantages of the proposed method include enhanced selectivity and sensitivity. Without prior enrichment of the analytes, limits of detection down to 2 × 10-9 M (20 fmol) may be reached for selected phenols, e.g., for 4-octylphenol, 4-ethylphenol, and 4-(1-indanyl)phenol. Only readily available instrumentation is required for these measurements.
    Original languageUndefined
    Pages (from-to)922-926
    JournalAnalytical chemistry
    Volume75
    Issue number4
    DOIs
    Publication statusPublished - 2003

    Keywords

    • METIS-216429
    • IR-72268

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