Liver-tumor mimics as a potential translational framework for planning and testing irreversible electroporation with multiple electrodes

Adriana Leticia Vera-Tizatl*, Regine van der Hee, Jeroen Cornelissen, Claudia Elizabeth Vera-Tizatl, Momen Abayazid, Jurgen J. Fütterer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
6 Downloads (Pure)

Abstract

Irreversible electroporation (IRE) has emerged as an appealing non-ionizing, non-thermal ablation therapy, independent of antineoplastic drugs. Limited but successful outcomes in IRE conducted in vivo, in small focal hepatocellular carcinomas (HCC), have been reported. Nonetheless, the electric parameters of IRE are usually delivered in an unplanned manner. This work investigates the integration of computational modeling to hydrogels mimicking the HCC microenvironment, as a powerful framework to: circumvent ethical concerns of in vivo experimentation; safely tune the electric parameters reaching the IRE electric field threshold; and propel the translation of IRE as a routine clinical alternative to the treatment of HCC. Therefore, a parametric study served to evaluate the effects of the pulse amplitude, the number of pulses and electrodes, the treatment time, the hydrogel–tumor size, and the cell type. The ablation extent was surveyed by confocal microscopy and magnetic resonance imaging (MRI) in cylindrical and realistic tumor-shaped hydrogels, respectively. A large ablation (70%–100%) was verified in all constructs.

Original languageEnglish
Article numbere10607
JournalBioengineering and Translational Medicine
Volume9
Issue number1
Early online date23 Nov 2023
DOIs
Publication statusPublished - Jan 2024

Keywords

  • co-cultured hydrogels
  • computational treatment planning
  • Hep-G2
  • hepatocellular carcinoma
  • HUVEC
  • hydrogel tumor

Fingerprint

Dive into the research topics of 'Liver-tumor mimics as a potential translational framework for planning and testing irreversible electroporation with multiple electrodes'. Together they form a unique fingerprint.

Cite this