# Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

Tijmen A. G. Hageman, Marc P. Pichel, Per A. Löthman, Jiung Cho, Miri Choi, Nuriye Korkmaz, Andreas Manz, Leon Abelmann

Research output: Working paperProfessional

### Abstract

We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.
Original language English 7 Published - 21 Feb 2019

### Publication series

Name Arxiv.org

bacterium
magnetic field
parameter

### Keywords

• physics.bio-ph

### Cite this

Hageman, T. A. G., Pichel, M. P., Löthman, P. A., Cho, J., Choi, M., Korkmaz, N., ... Abelmann, L. (2019). Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. (1902.08033v1 ed.) (Arxiv.org).
Hageman, Tijmen A. G. ; Pichel, Marc P. ; Löthman, Per A. ; Cho, Jiung ; Choi, Miri ; Korkmaz, Nuriye ; Manz, Andreas ; Abelmann, Leon. / Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. 1902.08033v1. ed. 2019. (Arxiv.org).
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title = "Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel",
abstract = "We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.",
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author = "Hageman, {Tijmen A. G.} and Pichel, {Marc P.} and L{\"o}thman, {Per A.} and Jiung Cho and Miri Choi and Nuriye Korkmaz and Andreas Manz and Leon Abelmann",
note = "7 pages, 11 figures",
year = "2019",
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language = "English",
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Hageman, TAG, Pichel, MP, Löthman, PA, Cho, J, Choi, M, Korkmaz, N, Manz, A & Abelmann, L 2019 'Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel' Arxiv.org, 1902.08033v1 edn.

Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. / Hageman, Tijmen A. G.; Pichel, Marc P.; Löthman, Per A.; Cho, Jiung; Choi, Miri; Korkmaz, Nuriye; Manz, Andreas; Abelmann, Leon.

1902.08033v1. ed. 2019. (Arxiv.org).

Research output: Working paperProfessional

TY - UNPB

T1 - Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

AU - Hageman, Tijmen A. G.

AU - Pichel, Marc P.

AU - Löthman, Per A.

AU - Cho, Jiung

AU - Choi, Miri

AU - Korkmaz, Nuriye

AU - Manz, Andreas

AU - Abelmann, Leon

N1 - 7 pages, 11 figures

PY - 2019/2/21

Y1 - 2019/2/21

N2 - We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

AB - We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

KW - physics.bio-ph

UR - https://arxiv.org/abs/1902.08033

M3 - Working paper

T3 - Arxiv.org

BT - Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

ER -

Hageman TAG, Pichel MP, Löthman PA, Cho J, Choi M, Korkmaz N et al. Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. 1902.08033v1 ed. 2019 Feb 21. (Arxiv.org).