Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

Tijmen A. G. Hageman, Marc P. Pichel, Per A. Löthman, Jiung Cho, Miri Choi, Nuriye Korkmaz, Andreas Manz, Leon Abelmann

Research output: Working paperProfessional

Abstract

We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.
Original languageEnglish
Number of pages7
Publication statusPublished - 21 Feb 2019

Publication series

NameArxiv.org

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bacterium
magnetic field
parameter

Keywords

  • physics.bio-ph

Cite this

Hageman, T. A. G., Pichel, M. P., Löthman, P. A., Cho, J., Choi, M., Korkmaz, N., ... Abelmann, L. (2019). Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. (1902.08033v1 ed.) (Arxiv.org).
Hageman, Tijmen A. G. ; Pichel, Marc P. ; Löthman, Per A. ; Cho, Jiung ; Choi, Miri ; Korkmaz, Nuriye ; Manz, Andreas ; Abelmann, Leon. / Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. 1902.08033v1. ed. 2019. (Arxiv.org).
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abstract = "We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.",
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Hageman, TAG, Pichel, MP, Löthman, PA, Cho, J, Choi, M, Korkmaz, N, Manz, A & Abelmann, L 2019 'Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel' Arxiv.org, 1902.08033v1 edn.

Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. / Hageman, Tijmen A. G.; Pichel, Marc P.; Löthman, Per A.; Cho, Jiung; Choi, Miri; Korkmaz, Nuriye; Manz, Andreas; Abelmann, Leon.

1902.08033v1. ed. 2019. (Arxiv.org).

Research output: Working paperProfessional

TY - UNPB

T1 - Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

AU - Hageman, Tijmen A. G.

AU - Pichel, Marc P.

AU - Löthman, Per A.

AU - Cho, Jiung

AU - Choi, Miri

AU - Korkmaz, Nuriye

AU - Manz, Andreas

AU - Abelmann, Leon

N1 - 7 pages, 11 figures

PY - 2019/2/21

Y1 - 2019/2/21

N2 - We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

AB - We controlled and observed individual magnetotactic bacteria (Magnetospirillum gryphiswaldense) inside a 5 {\mu}m high microfluidic channel for over four hours. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25 nm/s$^2$. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

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BT - Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

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Hageman TAG, Pichel MP, Löthman PA, Cho J, Choi M, Korkmaz N et al. Long term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. 1902.08033v1 ed. 2019 Feb 21. (Arxiv.org).