Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

Tijmen A.G. Hageman, Marc P. Pichel, Per A. Löthman, Jiung Cho, Miri Choi, Nuriye Korkmaz, Andreas Manz, Leon Abelmann*

*Corresponding author for this work

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    Abstract

    We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a 5μm-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25nm/s2. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

    Original languageEnglish
    Pages (from-to)1427-1433
    Number of pages7
    JournalArchives of Microbiology
    Volume201
    Issue number10
    Early online date14 Aug 2019
    DOIs
    Publication statusPublished - 1 Dec 2019

    Keywords

    • Bacteria
    • Magneto-tactic
    • Microfluidic
    • Velocity

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  • Cite this

    Hageman, T. A. G., Pichel, M. P., Löthman, P. A., Cho, J., Choi, M., Korkmaz, N., ... Abelmann, L. (2019). Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel. Archives of Microbiology, 201(10), 1427-1433. https://doi.org/10.1007/s00203-019-01713-0