Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

Tijmen A.G. Hageman; Marc P. Pichel; Per A. Löthman; Jiung Cho; Miri Choi; Nuriye Korkmaz; Andreas Manz; Leon Abelmann

doi:10.1007/s00203-019-01713-0

Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

Tijmen A.G. Hageman
, Marc P. Pichel
, Per A. Löthman
, Jiung Cho
, Miri Choi
, Nuriye Korkmaz
, Andreas Manz
, Leon Abelmann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

139 Downloads (Pure)

Abstract

We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a 5μm-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25nm/s². After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

Original language	English
Pages (from-to)	1427-1433
Number of pages	7
Journal	Archives of Microbiology
Volume	201
Issue number	10
Early online date	14 Aug 2019
DOIs	https://doi.org/10.1007/s00203-019-01713-0
Publication status	Published - 1 Dec 2019

Keywords

Bacteria
Magneto-tactic
Microfluidic
Velocity

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Hageman2019_Article_Long-termObservationOfMagnetosFinal published version, 1.82 MBLicence: CC BY

Cite this

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title = "Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel",

abstract = "We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a 5μm-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25nm/s2. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.",

keywords = "Bacteria, Magneto-tactic, Microfluidic, Velocity",

author = "Hageman, \{Tijmen A.G.\} and Pichel, \{Marc P.\} and L{\"o}thman, \{Per A.\} and Jiung Cho and Miri Choi and Nuriye Korkmaz and Andreas Manz and Leon Abelmann",

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doi = "10.1007/s00203-019-01713-0",

language = "English",

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TY - JOUR

T1 - Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

AU - Hageman, Tijmen A.G.

AU - Pichel, Marc P.

AU - Löthman, Per A.

AU - Cho, Jiung

AU - Choi, Miri

AU - Korkmaz, Nuriye

AU - Manz, Andreas

AU - Abelmann, Leon

PY - 2019/12/1

Y1 - 2019/12/1

N2 - We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a 5μm-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25nm/s2. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

AB - We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a 5μm-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about 25nm/s2. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.

KW - Bacteria

KW - Magneto-tactic

KW - Microfluidic

KW - Velocity

UR - https://www.scopus.com/pages/publications/85070717805

U2 - 10.1007/s00203-019-01713-0

DO - 10.1007/s00203-019-01713-0

M3 - Article

AN - SCOPUS:85070717805

SN - 0302-8933

VL - 201

SP - 1427

EP - 1433

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 10

ER -

Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel

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