Looking at the photodynamics of individual fluorescent molecules and proteins

M.F. Garcia Parajo; J.A. Veerman; L. Kuipers; N.F. van Hulst

doi:10.1351/pac200173030431

Looking at the photodynamics of individual fluorescent molecules and proteins

M.F. Garcia Parajo, J.A. Veerman, L. Kuipers, N.F. van Hulst

Optical Sciences

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Abstract

The photodynamics of individual molecules and fluorescent proteins has been investigated in real time. In the case of organic molecules, both the triplet state lifetime and intersystem crossing yield appear to vary in time and space. In the case of autofluorescent proteins, light-driven "on-off" fluorescence emission has been observed, with a behavior that is consistent with the existence of a long-lived dark state.

Original language	Undefined
Pages (from-to)	431-434
Number of pages	4
Journal	Pure and applied chemistry
Volume	73
Issue number	3
DOIs	https://doi.org/10.1351/pac200173030431
Publication status	Published - 2001

Keywords

IR-36482
METIS-202082

Access to Document

10.1351/pac200173030431

Cite this

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title = "Looking at the photodynamics of individual fluorescent molecules and proteins",

abstract = "The photodynamics of individual molecules and fluorescent proteins has been investigated in real time. In the case of organic molecules, both the triplet state lifetime and intersystem crossing yield appear to vary in time and space. In the case of autofluorescent proteins, light-driven {"}on-off{"} fluorescence emission has been observed, with a behavior that is consistent with the existence of a long-lived dark state.",

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year = "2001",

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pages = "431--434",

journal = "Pure and applied chemistry",

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T1 - Looking at the photodynamics of individual fluorescent molecules and proteins

AU - Garcia Parajo, M.F.

AU - Veerman, J.A.

AU - Kuipers, L.

AU - van Hulst, N.F.

PY - 2001

Y1 - 2001

N2 - The photodynamics of individual molecules and fluorescent proteins has been investigated in real time. In the case of organic molecules, both the triplet state lifetime and intersystem crossing yield appear to vary in time and space. In the case of autofluorescent proteins, light-driven "on-off" fluorescence emission has been observed, with a behavior that is consistent with the existence of a long-lived dark state.

AB - The photodynamics of individual molecules and fluorescent proteins has been investigated in real time. In the case of organic molecules, both the triplet state lifetime and intersystem crossing yield appear to vary in time and space. In the case of autofluorescent proteins, light-driven "on-off" fluorescence emission has been observed, with a behavior that is consistent with the existence of a long-lived dark state.

KW - IR-36482

KW - METIS-202082

U2 - 10.1351/pac200173030431

DO - 10.1351/pac200173030431

M3 - Article

SN - 0033-4545

VL - 73

SP - 431

EP - 434

JO - Pure and applied chemistry

JF - Pure and applied chemistry

IS - 3

ER -