Low pressure microfluidic-based DNA fragmentation

Lingling Shui, Wouter Sparreboom, Johan G. Bomer, Mingliang Jin, Edwin Carlen, Albert van den Berg

    Research output: Contribution to conferencePaper

    Abstract

    We report a low-pressure microfluidic deoxyribonucleic acid (DNA) fragmentation device based on a combination of me-chanical hydrodynamic shearing and low temperature sample heating. Conventional DNA fragmentation based on hydrody-namic shearing is capable of achieving fragment lengths (FL) < 10k bp with pressures ΔP > 1 bar. The application of a small amount of additional thermal energy to the buffer medium results in ~10x reduction in pressure requirement resulting in FL~6k-8k bp with ΔP~1-0.1 bar with sample temperature Ts~45º C
    Original languageUndefined
    Pages1191-1193
    Number of pages3
    Publication statusPublished - 2 Oct 2011
    Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 - Seattle, United States
    Duration: 2 Oct 20116 Oct 2011
    Conference number: 15
    https://microtasconferences.org/microtas2011/

    Conference

    Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySeattle
    Period2/10/116/10/11
    Internet address

    Keywords

    • EWI-20894
    • IR-78659

    Cite this

    Shui, L., Sparreboom, W., Bomer, J. G., Jin, M., Carlen, E., & van den Berg, A. (2011). Low pressure microfluidic-based DNA fragmentation. 1191-1193. Paper presented at 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011, Seattle, United States.