Lowering caveolin-1 expression in human vascular cells inhibits signal transduction in response to shear stress

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Abstract

Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm2 for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.
Original languageUndefined
Pages (from-to)-
Number of pages9
JournalJournal of cell biology
Issue number532432
DOIs
Publication statusPublished - 2009

Keywords

  • IR-74808
  • METIS-264529

Cite this

@article{cdbcdb1c3b10423995fedafc5c762d86,
title = "Lowering caveolin-1 expression in human vascular cells inhibits signal transduction in response to shear stress",
abstract = "Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm2 for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.",
keywords = "IR-74808, METIS-264529",
author = "{van der Meer}, {Andries Dirk} and Marloes Kamphuis and Poot, {Andreas A.} and Jan Feijen and I. Vermes",
note = "Open access article",
year = "2009",
doi = "10.1155/2009/532432",
language = "Undefined",
pages = "--",
journal = "Journal of cell biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "532432",

}

Lowering caveolin-1 expression in human vascular cells inhibits signal transduction in response to shear stress. / van der Meer, Andries Dirk; Kamphuis, Marloes; Poot, Andreas A.; Feijen, Jan; Vermes, I.

In: Journal of cell biology, No. 532432, 2009, p. -.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Lowering caveolin-1 expression in human vascular cells inhibits signal transduction in response to shear stress

AU - van der Meer, Andries Dirk

AU - Kamphuis, Marloes

AU - Poot, Andreas A.

AU - Feijen, Jan

AU - Vermes, I.

N1 - Open access article

PY - 2009

Y1 - 2009

N2 - Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm2 for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.

AB - Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm2 for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.

KW - IR-74808

KW - METIS-264529

U2 - 10.1155/2009/532432

DO - 10.1155/2009/532432

M3 - Article

SP - -

JO - Journal of cell biology

JF - Journal of cell biology

SN - 0021-9525

IS - 532432

ER -