Luminescence thermometry for In situ temperature measurements in microfluidic devices

Robin G. Geitenbeek (Corresponding Author), Jeroen C. Vollenbroek (Corresponding Author), Hannah M.H. Weijgertze, Corentin B.M. Tregouet, Anne Eva Nieuwelink, Chris L. Kennedy, Bert M. Weckhuysen, Detlef Lohse, Alfons Van Blaaderen, Albert Van Den Berg, Mathieu Odijk, Andries Meijerink

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Abstract

Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.

Original languageEnglish
Pages (from-to)1236-1246
Number of pages11
JournalLab on a chip
Volume19
Issue number7
DOIs
Publication statusE-pub ahead of print/First online - 25 Feb 2019

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Lab-On-A-Chip Devices
Thermometry
Luminescence
Microfluidics
Temperature measurement
Temperature
Lab-on-a-chip
Confocal microscopy
Polymerase chain reaction
Optical resolving power
Temperature sensors
Electrophoresis
Temperature control
Thermal gradients
Fiber optics
Coloring Agents
Dyes
Confocal Microscopy
Nanoparticles
Detectors

Cite this

Geitenbeek, R. G., Vollenbroek, J. C., Weijgertze, H. M. H., Tregouet, C. B. M., Nieuwelink, A. E., Kennedy, C. L., ... Meijerink, A. (2019). Luminescence thermometry for In situ temperature measurements in microfluidic devices. Lab on a chip, 19(7), 1236-1246. https://doi.org/10.1039/c8lc01292j
Geitenbeek, Robin G. ; Vollenbroek, Jeroen C. ; Weijgertze, Hannah M.H. ; Tregouet, Corentin B.M. ; Nieuwelink, Anne Eva ; Kennedy, Chris L. ; Weckhuysen, Bert M. ; Lohse, Detlef ; Van Blaaderen, Alfons ; Van Den Berg, Albert ; Odijk, Mathieu ; Meijerink, Andries. / Luminescence thermometry for In situ temperature measurements in microfluidic devices. In: Lab on a chip. 2019 ; Vol. 19, No. 7. pp. 1236-1246.
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abstract = "Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.",
author = "Geitenbeek, {Robin G.} and Vollenbroek, {Jeroen C.} and Weijgertze, {Hannah M.H.} and Tregouet, {Corentin B.M.} and Nieuwelink, {Anne Eva} and Kennedy, {Chris L.} and Weckhuysen, {Bert M.} and Detlef Lohse and {Van Blaaderen}, Alfons and {Van Den Berg}, Albert and Mathieu Odijk and Andries Meijerink",
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Geitenbeek, RG, Vollenbroek, JC, Weijgertze, HMH, Tregouet, CBM, Nieuwelink, AE, Kennedy, CL, Weckhuysen, BM, Lohse, D, Van Blaaderen, A, Van Den Berg, A, Odijk, M & Meijerink, A 2019, 'Luminescence thermometry for In situ temperature measurements in microfluidic devices' Lab on a chip, vol. 19, no. 7, pp. 1236-1246. https://doi.org/10.1039/c8lc01292j

Luminescence thermometry for In situ temperature measurements in microfluidic devices. / Geitenbeek, Robin G. (Corresponding Author); Vollenbroek, Jeroen C. (Corresponding Author); Weijgertze, Hannah M.H.; Tregouet, Corentin B.M.; Nieuwelink, Anne Eva; Kennedy, Chris L.; Weckhuysen, Bert M.; Lohse, Detlef; Van Blaaderen, Alfons; Van Den Berg, Albert; Odijk, Mathieu; Meijerink, Andries.

In: Lab on a chip, Vol. 19, No. 7, 25.02.2019, p. 1236-1246.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Luminescence thermometry for In situ temperature measurements in microfluidic devices

AU - Geitenbeek, Robin G.

AU - Vollenbroek, Jeroen C.

AU - Weijgertze, Hannah M.H.

AU - Tregouet, Corentin B.M.

AU - Nieuwelink, Anne Eva

AU - Kennedy, Chris L.

AU - Weckhuysen, Bert M.

AU - Lohse, Detlef

AU - Van Blaaderen, Alfons

AU - Van Den Berg, Albert

AU - Odijk, Mathieu

AU - Meijerink, Andries

PY - 2019/2/25

Y1 - 2019/2/25

N2 - Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.

AB - Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.

U2 - 10.1039/c8lc01292j

DO - 10.1039/c8lc01292j

M3 - Article

VL - 19

SP - 1236

EP - 1246

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 7

ER -

Geitenbeek RG, Vollenbroek JC, Weijgertze HMH, Tregouet CBM, Nieuwelink AE, Kennedy CL et al. Luminescence thermometry for In situ temperature measurements in microfluidic devices. Lab on a chip. 2019 Feb 25;19(7):1236-1246. https://doi.org/10.1039/c8lc01292j