Lysozyme self-assembles into amyloid networks that support cartilage tissue formation

M. van Dalen; J. Post; M. Karperien; Mireille M.A.E. Claessens

doi:10.1016/j.joca.2016.01.848

Abstract

Purpose: No cure is available for repair of damaged cartilage. Once damaged, cartilage will continue to degenerate, resulting in immobile and painful joints. Treatments are limited to symptom relief, while tissue engineering approaches fail to produce cartilage comparable to native articular cartilage in terms of strength and modulus. In this project, we investigated the use of self-assembling proteins as scaffold material for cartilage tissue engineering. These so-called amy-loid networks consist of amyloid fibrils forming physical cross-links. The fibrils self-assemble from proteins by forming inter-protein B-sheets.
Amyloid networks resemble the extracellular matrix of cartilage
since the strength and Young’s modulus of the fibrils is comparable to
those of collagen and the networks are hydrogels. We therefore
hypothesized that amyloid networks can be used as scaffold material for
cartilage tissue engineering.

Original language	English
Article number	794
Pages (from-to)	S465
Journal	Osteoarthritis and cartilage
Volume	24
Issue number	supplement 1
DOIs	https://doi.org/10.1016/j.joca.2016.01.848
Publication status	Published - Apr 2016
Event	2016 OARSI World Congress on Osteoarthrtis - Amsterdam RAI, Amsterdam, Netherlands Duration: 31 Mar 2016 → 3 Apr 2016

Keywords

IR-103806
METIS-314882

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10.1016/j.joca.2016.01.848

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title = "Lysozyme self-assembles into amyloid networks that support cartilage tissue formation",

abstract = "Purpose: No cure is available for repair of damaged cartilage. Once damaged, cartilage will continue to degenerate, resulting in immobile and painful joints. Treatments are limited to symptom relief, while tissue engineering approaches fail to produce cartilage comparable to native articular cartilage in terms of strength and modulus. In this project, we investigated the use of self-assembling proteins as scaffold material for cartilage tissue engineering. These so-called amy-loid networks consist of amyloid fibrils forming physical cross-links. The fibrils self-assemble from proteins by forming inter-protein B-sheets.Amyloid networks resemble the extracellular matrix of cartilagesince the strength and Young{\textquoteright}s modulus of the fibrils is comparable tothose of collagen and the networks are hydrogels. We thereforehypothesized that amyloid networks can be used as scaffold material forcartilage tissue engineering.",

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AU - van Dalen, M.

AU - Post, J.

AU - Karperien, M.

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PY - 2016/4

Y1 - 2016/4

N2 - Purpose: No cure is available for repair of damaged cartilage. Once damaged, cartilage will continue to degenerate, resulting in immobile and painful joints. Treatments are limited to symptom relief, while tissue engineering approaches fail to produce cartilage comparable to native articular cartilage in terms of strength and modulus. In this project, we investigated the use of self-assembling proteins as scaffold material for cartilage tissue engineering. These so-called amy-loid networks consist of amyloid fibrils forming physical cross-links. The fibrils self-assemble from proteins by forming inter-protein B-sheets.Amyloid networks resemble the extracellular matrix of cartilagesince the strength and Young’s modulus of the fibrils is comparable tothose of collagen and the networks are hydrogels. We thereforehypothesized that amyloid networks can be used as scaffold material forcartilage tissue engineering.

AB - Purpose: No cure is available for repair of damaged cartilage. Once damaged, cartilage will continue to degenerate, resulting in immobile and painful joints. Treatments are limited to symptom relief, while tissue engineering approaches fail to produce cartilage comparable to native articular cartilage in terms of strength and modulus. In this project, we investigated the use of self-assembling proteins as scaffold material for cartilage tissue engineering. These so-called amy-loid networks consist of amyloid fibrils forming physical cross-links. The fibrils self-assemble from proteins by forming inter-protein B-sheets.Amyloid networks resemble the extracellular matrix of cartilagesince the strength and Young’s modulus of the fibrils is comparable tothose of collagen and the networks are hydrogels. We thereforehypothesized that amyloid networks can be used as scaffold material forcartilage tissue engineering.

KW - IR-103806

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DO - 10.1016/j.joca.2016.01.848

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Lysozyme self-assembles into amyloid networks that support cartilage tissue formation

Abstract

Keywords

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