Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing

Jean-Philippe Frimat, M. Bronkhorst, B. de Wagenaar, Johan G. Bomer, Ferdinand van der Heijden, Albert van den Berg, Loes Irene Segerink

    Research output: Contribution to journalArticleAcademicpeer-review

    16 Citations (Scopus)

    Abstract

    In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes
    Original languageUndefined
    Pages (from-to)2635-2641
    Number of pages7
    JournalLab on a chip
    Volume15
    Issue number15
    DOIs
    Publication statusPublished - 7 Aug 2014

    Keywords

    • EWI-25201
    • IR-92317
    • METIS-306078

    Cite this

    @article{4c775ed158624a0f8b30f4a460ca9039,
    title = "Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing",
    abstract = "In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90{\%} while retaining 100{\%} viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes",
    keywords = "EWI-25201, IR-92317, METIS-306078",
    author = "Jean-Philippe Frimat and M. Bronkhorst and {de Wagenaar}, B. and Bomer, {Johan G.} and {van der Heijden}, Ferdinand and {van den Berg}, Albert and Segerink, {Loes Irene}",
    note = "eemcs-eprint-25201",
    year = "2014",
    month = "8",
    day = "7",
    doi = "10.1039/C4LC00050A",
    language = "Undefined",
    volume = "15",
    pages = "2635--2641",
    journal = "Lab on a chip",
    issn = "1473-0197",
    publisher = "Royal Society of Chemistry",
    number = "15",

    }

    Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing. / Frimat, Jean-Philippe; Bronkhorst, M.; de Wagenaar, B.; Bomer, Johan G.; van der Heijden, Ferdinand; van den Berg, Albert; Segerink, Loes Irene.

    In: Lab on a chip, Vol. 15, No. 15, 07.08.2014, p. 2635-2641.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing

    AU - Frimat, Jean-Philippe

    AU - Bronkhorst, M.

    AU - de Wagenaar, B.

    AU - Bomer, Johan G.

    AU - van der Heijden, Ferdinand

    AU - van den Berg, Albert

    AU - Segerink, Loes Irene

    N1 - eemcs-eprint-25201

    PY - 2014/8/7

    Y1 - 2014/8/7

    N2 - In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes

    AB - In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes

    KW - EWI-25201

    KW - IR-92317

    KW - METIS-306078

    U2 - 10.1039/C4LC00050A

    DO - 10.1039/C4LC00050A

    M3 - Article

    VL - 15

    SP - 2635

    EP - 2641

    JO - Lab on a chip

    JF - Lab on a chip

    SN - 1473-0197

    IS - 15

    ER -