Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing

Jean-Philippe Frimat, M. Bronkhorst, B. de Wagenaar, Johan G. Bomer, Ferdinand van der Heijden, Albert van den Berg, Loes Irene Segerink

    Research output: Contribution to journalArticleAcademicpeer-review

    16 Citations (Scopus)

    Abstract

    In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes
    Original languageUndefined
    Pages (from-to)2635-2641
    Number of pages7
    JournalLab on a chip
    Volume15
    Issue number15
    DOIs
    Publication statusPublished - 7 Aug 2014

    Keywords

    • EWI-25201
    • IR-92317
    • METIS-306078

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