Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing

Jean-Philippe Frimat, M. Bronkhorst, B. de Wagenaar, Johan G. Bomer, Ferdinand van der Heijden, Albert van den Berg, Loes Irene Segerink

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes
Original languageUndefined
Pages (from-to)2635-2641
Number of pages7
JournalLab on a chip
Volume15
Issue number15
DOIs
Publication statusPublished - 7 Aug 2014

Keywords

  • EWI-25201
  • IR-92317
  • METIS-306078

Cite this

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title = "Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing",
abstract = "In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90{\%} while retaining 100{\%} viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes",
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author = "Jean-Philippe Frimat and M. Bronkhorst and {de Wagenaar}, B. and Bomer, {Johan G.} and {van der Heijden}, Ferdinand and {van den Berg}, Albert and Segerink, {Loes Irene}",
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doi = "10.1039/C4LC00050A",
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pages = "2635--2641",
journal = "Lab on a chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
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Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing. / Frimat, Jean-Philippe; Bronkhorst, M.; de Wagenaar, B.; Bomer, Johan G.; van der Heijden, Ferdinand; van den Berg, Albert; Segerink, Loes Irene.

In: Lab on a chip, Vol. 15, No. 15, 07.08.2014, p. 2635-2641.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing

AU - Frimat, Jean-Philippe

AU - Bronkhorst, M.

AU - de Wagenaar, B.

AU - Bomer, Johan G.

AU - van der Heijden, Ferdinand

AU - van den Berg, Albert

AU - Segerink, Loes Irene

N1 - eemcs-eprint-25201

PY - 2014/8/7

Y1 - 2014/8/7

N2 - In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes

AB - In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes

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DO - 10.1039/C4LC00050A

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JO - Lab on a chip

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